Forsyditerpenes A–O, CC-type clerodane and aromatic abietane diterpenoids with anti-inflammatory activities from the seeds of Forsythia suspensa

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-06-12 DOI:10.1016/j.fitote.2025.106675

Hui-Ying Li , Meng-Yu Bao , Hao-Ming Xiong , Can-Can Wang , Li-Ping Bai , Wei Zhang , Cheng-Yu Chen , Zhi-Hong Jiang , Guo-Yuan Zhu

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Abstract

Diterpenoids, the bioactive terpenoids of many medicinal plants, have not been reported in the seeds of Forsythia suspensa. In this study, twelve undescribed clerodane (1−12) and three new aromatic abietane (13–15) diterpenoids, named forsyditerpenes A–O, were isolated from the seeds of Forsythia suspensa. Among them, forsyditerpenes A–L (1–12) have rear CC-type clerodane skeleton, which possess the cis configuration of the A/B ring junction and the α-oriented C-17 and C-20. Furthermore, forsyditerpenes N (14) and O (15) are rearranged ent-abietane diterpenoids. Structural characterization was achieved by a combination of NMR, HR-ESI-MS, and X-ray diffraction. Forsyditerpenes C (3) and D (4) showed inhibitory effects of LPS-induced NO release with IC₅₀ values of 26.2 and 12.8 μM, respectively, which should be partly achieved through inhibiting iNOS protein expression in RAW264.7 cells.

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连翘种子中具有抗炎活性的连翘二萜A-O、cc型氯烷和芳香枞烷二萜。

二萜是许多药用植物的活性萜类化合物，在连翘（Forsythia suspensa）种子中尚未发现。从连翘（forsydiiterpenes）种子中分离得到12个未描述的氯烷（1-12）和3个新的芳香枞烷（13-15）二萜，命名为连翘二萜A-O。其中，连翘二萜A- l（1-12）具有后cc型氯烷骨架，具有A/B环结的顺式构型和α取向的C-17和C-20。此外，连翘二萜N（14）和O（15）是重排的对枞烷二萜。结构表征是通过NMR， HR-ESI-MS和x射线衍射的组合来实现的。Forsyditerpenes C(3)和D(4)对lps诱导的NO释放有抑制作用，IC50值分别为26.2和12.8 μM，部分可能是通过抑制RAW264.7细胞中iNOS蛋白的表达来实现的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.