A New Vaccine Candidate Expressing JUNV GP1-GP2 Against Argentine Hemorrhagic Fever Based on Baculovirus Surface Display.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-06-13 DOI:10.1007/s00284-025-04320-z

Carla Tomatis, Mara C A M Aguiar, Nancy Charo, Silvia Aquila, Pablo D Thomas, Paula N Arrías, Matías L Pidre, Víctor Romanowski, Laura Riera, Julia Brignone, Andrea Maiza, Eugenio Antonio Carrera Silva, María Florencia Ferrer, Ricardo Martín Gómez

{"title":"A New Vaccine Candidate Expressing JUNV GP1-GP2 Against Argentine Hemorrhagic Fever Based on Baculovirus Surface Display.","authors":"Carla Tomatis, Mara C A M Aguiar, Nancy Charo, Silvia Aquila, Pablo D Thomas, Paula N Arrías, Matías L Pidre, Víctor Romanowski, Laura Riera, Julia Brignone, Andrea Maiza, Eugenio Antonio Carrera Silva, María Florencia Ferrer, Ricardo Martín Gómez","doi":"10.1007/s00284-025-04320-z","DOIUrl":null,"url":null,"abstract":"<p><p>With the aim of developing a new vaccine candidate using a recombinant baculovirus (rBV) expressing the Junín virus (JUNV) GP1 and GP2 proteins on its surface, a pBacPAK9 vector containing the BV-GP64 signal peptide, the JUNV GP1-GP2 and the transmembrane domain of the vesicular stomatitis virus G (VSV-G) protein were synthesized. This plasmid was co-transfected with the bApGOZA bacmid into Sf9 cells. The rBV was recovered from the supernatant. Protein expression was confirmed with a human serum by Western blot and with an anti-GPC monoclonal antibody by IFI staining and virometry. In an ELISA test, mice immunized with rBV exhibited specific antibody levels that also showed neutralizing properties in a plaque reduction test compared to controls. Guinea pigs immunized with rBV and challenged with JUNV showed partial protection compared to the Candid#1 vaccine. We have developed an rBV surface display platform to validate a potential JUNV vaccine candidate.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 8","pages":"334"},"PeriodicalIF":2.3000,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-025-04320-z","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

With the aim of developing a new vaccine candidate using a recombinant baculovirus (rBV) expressing the Junín virus (JUNV) GP1 and GP2 proteins on its surface, a pBacPAK9 vector containing the BV-GP64 signal peptide, the JUNV GP1-GP2 and the transmembrane domain of the vesicular stomatitis virus G (VSV-G) protein were synthesized. This plasmid was co-transfected with the bApGOZA bacmid into Sf9 cells. The rBV was recovered from the supernatant. Protein expression was confirmed with a human serum by Western blot and with an anti-GPC monoclonal antibody by IFI staining and virometry. In an ELISA test, mice immunized with rBV exhibited specific antibody levels that also showed neutralizing properties in a plaque reduction test compared to controls. Guinea pigs immunized with rBV and challenged with JUNV showed partial protection compared to the Candid#1 vaccine. We have developed an rBV surface display platform to validate a potential JUNV vaccine candidate.

查看原文本刊更多论文

基于杆状病毒表面展示表达JUNV GP1-GP2的阿根廷出血热新候选疫苗

为了利用表面表达Junín病毒（JUNV） GP1和GP2蛋白的重组杆状病毒（rBV）开发新的候选疫苗，合成了含有BV-GP64信号肽、JUNV GP1-GP2和水疱性口炎病毒G （VSV-G）蛋白跨膜结构域的pBacPAK9载体。将该质粒与bApGOZA bacmid共转染至Sf9细胞。从上清液中回收rBV。Western blot法检测人血清蛋白表达，IFI染色和病毒测定法检测抗gpc单克隆抗体表达。在ELISA测试中，与对照组相比，接种rBV的小鼠表现出特异性抗体水平，在斑块减少测试中也表现出中和特性。与Candid 1号疫苗相比，接种rBV和JUNV的豚鼠表现出部分保护作用。我们已经开发了一个rBV表面展示平台来验证潜在的JUNV候选疫苗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.