Identification of antifungal metabolites from a soil-derived Streptomyces strain active against Fusarium graminearum using genome mining.

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2025-06-13 DOI:10.1007/s10529-025-03605-4

Xingyu Liu, Lubna Rahman, Yang Zhang, Rui Guo, Min Zhang, Wei Zhou

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Abstract

Microbes derived from soil environments are well-recognized as prolific producers of bioactive secondary metabolites, making significant contributions to the advancement of antibiotic research. In this study, we isolated a soil-derived Streptomyces strain, designated Streptomyces sp. AHU1, which exhibited potent antifungal activity against Fusarium graminearum, the primary causative agent of Fusarium head blight. The potential of Streptomyces sp. AHU1 as a novel phytopathogenic fungicide was validated through in vitro antifungal assays and infection bioassays conducted on wheat seedlings. Whole genome sequencing combined with anti-SMASH genome mining identified approximately 34 biosynthetic gene clusters (BGCs) within the genome of Streptomyces sp. AHU1. Targeted isolation and functional analysis of these BGCs revealed that the antifungal activity is primarily attributed to lucensomycin, a previously characterized antifungal compound. This study underscores the potential of Streptomyces sp. AHU1 as a promising agent for sustainable crop protection and highlights the effectiveness of integrating bioassay-based screening with genome mining for BGC characterization as a robust strategy for the discovery of novel phytopathogenic fungicides.

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一株土壤源链霉菌抗谷物镰刀菌代谢产物的基因组挖掘鉴定。

来自土壤环境的微生物被公认为是生物活性次生代谢物的多产生产者，对抗生素研究的进步做出了重大贡献。在这项研究中，我们分离到了一株土壤来源的链霉菌，命名为Streptomyces sp. AHU1，它对镰刀菌（Fusarium graminearum）有很强的抗真菌活性，镰刀菌是枯萎病的主要病原体。通过对小麦幼苗的体外抑菌试验和侵染生物试验，验证了链霉菌AHU1作为一种新型植物病原性杀菌剂的潜力。全基因组测序结合anti-SMASH基因组挖掘在链霉菌AHU1基因组中鉴定出约34个生物合成基因簇（bgc）。这些BGCs的靶向分离和功能分析表明，其抗真菌活性主要归因于lucensomycin，这是一种先前发现的抗真菌化合物。该研究强调了链霉菌（Streptomyces sp. AHU1）作为可持续作物保护剂的潜力，并强调了将基于生物测定的筛选与基因组挖掘结合起来进行BGC表征的有效性，这是发现新型植物病原杀菌剂的有力策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.