{"title":"Effects of gender, age and method variations on contractility in extremity lymphatic collectors using indocyanine green fluorescence lymphangiography","authors":"Mads Fich Lønnee, Mads Radmer Jensen, Bryan Haddock, Lene Simonsen, Tonny Karlsmark, Jens Bülow","doi":"10.1111/cpf.70017","DOIUrl":null,"url":null,"abstract":"<p>The objectives of this study were to examine how extremity lymphatic collector contraction frequency is influenced by gender, age, and methodological variations in indocyanine green (ICG) injection using near infrared fluorescence (NIRF) imaging in healthy adults. Ten women (19–46 years) and eight men (18–59 years) were examined with 30-min NIRF videos recorded at different time points 0–150 min after injection of ICG. Variations in ICG volume (0.1 mL vs. 0.3 mL), -concentration (1 mg/mL vs. 2.5 mg/mL), -injection site (hand vs. foot), -route of administration (subcutaneous vs. intradermal) were applied to all subjects. The primary outcome was mean contraction frequency with maximum contraction frequency and number of visualised extremity lymphatic collectors as secondary outcomes. The median of mean contraction frequencies for females and males were 0.42 min<sup>−1</sup> (IQR 0,19 min<sup>−1</sup>) and 0.25 min<sup>−1</sup> (IQR 0,15 min<sup>−1</sup>), respectively (<i>p</i> = 0.022). The median of maximum contraction frequencies for females and males were 0.70 min<sup>−1</sup> (IQR 0,26 min<sup>−1</sup>) and 0.34 min<sup>−1</sup> (IQR 0,15 min<sup>−1</sup>), respectively (<i>p</i> = 0.015). Neither age, method variations nor imaging delay had any significant effect on contraction frequencies. Number of visualised collectors increased slightly during 150 min (<i>p</i> = 0.02). Mean and maximum contraction frequencies were significantly higher in women compared to men. This is a novel finding that warrants verification in future studies. Contraction frequency measured with 30-min NIRF imaging using an ICG volume as little as 0.1 mL and a concentration of 1 mg/mL is seemingly robust and readily available. This method is recommendable for perturbation studies of lymphatic collector function and pathophysiology.</p>","PeriodicalId":10504,"journal":{"name":"Clinical Physiology and Functional Imaging","volume":"45 4","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Physiology and Functional Imaging","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/cpf.70017","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The objectives of this study were to examine how extremity lymphatic collector contraction frequency is influenced by gender, age, and methodological variations in indocyanine green (ICG) injection using near infrared fluorescence (NIRF) imaging in healthy adults. Ten women (19–46 years) and eight men (18–59 years) were examined with 30-min NIRF videos recorded at different time points 0–150 min after injection of ICG. Variations in ICG volume (0.1 mL vs. 0.3 mL), -concentration (1 mg/mL vs. 2.5 mg/mL), -injection site (hand vs. foot), -route of administration (subcutaneous vs. intradermal) were applied to all subjects. The primary outcome was mean contraction frequency with maximum contraction frequency and number of visualised extremity lymphatic collectors as secondary outcomes. The median of mean contraction frequencies for females and males were 0.42 min−1 (IQR 0,19 min−1) and 0.25 min−1 (IQR 0,15 min−1), respectively (p = 0.022). The median of maximum contraction frequencies for females and males were 0.70 min−1 (IQR 0,26 min−1) and 0.34 min−1 (IQR 0,15 min−1), respectively (p = 0.015). Neither age, method variations nor imaging delay had any significant effect on contraction frequencies. Number of visualised collectors increased slightly during 150 min (p = 0.02). Mean and maximum contraction frequencies were significantly higher in women compared to men. This is a novel finding that warrants verification in future studies. Contraction frequency measured with 30-min NIRF imaging using an ICG volume as little as 0.1 mL and a concentration of 1 mg/mL is seemingly robust and readily available. This method is recommendable for perturbation studies of lymphatic collector function and pathophysiology.
期刊介绍:
Clinical Physiology and Functional Imaging publishes reports on clinical and experimental research pertinent to human physiology in health and disease. The scope of the Journal is very broad, covering all aspects of the regulatory system in the cardiovascular, renal and pulmonary systems with special emphasis on methodological aspects. The focus for the journal is, however, work that has potential clinical relevance. The Journal also features review articles on recent front-line research within these fields of interest.
Covered by the major abstracting services including Current Contents and Science Citation Index, Clinical Physiology and Functional Imaging plays an important role in providing effective and productive communication among clinical physiologists world-wide.