Effects of gender, age and method variations on contractility in extremity lymphatic collectors using indocyanine green fluorescence lymphangiography

IF 1.3 4区 医学 Q4 PHYSIOLOGY
Mads Fich Lønnee, Mads Radmer Jensen, Bryan Haddock, Lene Simonsen, Tonny Karlsmark, Jens Bülow
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引用次数: 0

Abstract

The objectives of this study were to examine how extremity lymphatic collector contraction frequency is influenced by gender, age, and methodological variations in indocyanine green (ICG) injection using near infrared fluorescence (NIRF) imaging in healthy adults. Ten women (19–46 years) and eight men (18–59 years) were examined with 30-min NIRF videos recorded at different time points 0–150 min after injection of ICG. Variations in ICG volume (0.1 mL vs. 0.3 mL), -concentration (1 mg/mL vs. 2.5 mg/mL), -injection site (hand vs. foot), -route of administration (subcutaneous vs. intradermal) were applied to all subjects. The primary outcome was mean contraction frequency with maximum contraction frequency and number of visualised extremity lymphatic collectors as secondary outcomes. The median of mean contraction frequencies for females and males were 0.42 min−1 (IQR 0,19 min−1) and 0.25 min−1 (IQR 0,15 min−1), respectively (p = 0.022). The median of maximum contraction frequencies for females and males were 0.70 min−1 (IQR 0,26 min−1) and 0.34 min−1 (IQR 0,15 min−1), respectively (p = 0.015). Neither age, method variations nor imaging delay had any significant effect on contraction frequencies. Number of visualised collectors increased slightly during 150 min (p = 0.02). Mean and maximum contraction frequencies were significantly higher in women compared to men. This is a novel finding that warrants verification in future studies. Contraction frequency measured with 30-min NIRF imaging using an ICG volume as little as 0.1 mL and a concentration of 1 mg/mL is seemingly robust and readily available. This method is recommendable for perturbation studies of lymphatic collector function and pathophysiology.

性别、年龄和方法变化对使用吲哚菁绿荧光淋巴管造影的四肢淋巴收集器收缩性的影响
本研究的目的是利用近红外荧光(NIRF)成像技术研究健康成人注射吲哚菁绿(ICG)时,性别、年龄和方法变化对下肢淋巴收集器收缩频率的影响。10名女性(19-46岁)和8名男性(18-59岁)在注射ICG后0-150分钟的不同时间点记录30分钟NIRF视频。ICG体积(0.1 mL vs. 0.3 mL)、浓度(1mg /mL vs. 2.5 mg/mL)、注射部位(手vs.脚)、给药途径(皮下vs.皮内)的变化应用于所有受试者。主要结果是平均收缩频率,最大收缩频率和可见的四肢淋巴收集器数量作为次要结果。女性和男性的平均收缩频率中位数分别为0.42 min−1 (IQR 0,19 min−1)和0.25 min−1 (IQR 0,15 min−1)(p = 0.022)。女性和男性的最大收缩频率中位数分别为0.70 min−1 (IQR 0,26 min−1)和0.34 min−1 (IQR 0,15 min−1)(p = 0.015)。年龄、方法变化和成像延迟对收缩频率均无显著影响。在150分钟内,可视化收集器的数量略有增加(p = 0.02)。女性的平均和最大宫缩频率明显高于男性。这是一个新颖的发现,值得在未来的研究中验证。使用ICG体积低至0.1 mL,浓度为1mg /mL,用30分钟NIRF成像测量收缩频率似乎是可靠的,并且容易获得。该方法适用于淋巴收集功能和病理生理的摄动研究。
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来源期刊
CiteScore
3.40
自引率
5.60%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Clinical Physiology and Functional Imaging publishes reports on clinical and experimental research pertinent to human physiology in health and disease. The scope of the Journal is very broad, covering all aspects of the regulatory system in the cardiovascular, renal and pulmonary systems with special emphasis on methodological aspects. The focus for the journal is, however, work that has potential clinical relevance. The Journal also features review articles on recent front-line research within these fields of interest. Covered by the major abstracting services including Current Contents and Science Citation Index, Clinical Physiology and Functional Imaging plays an important role in providing effective and productive communication among clinical physiologists world-wide.
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