Metabolomic and microbial insights: Kai-Xin-San’s impact on Alzheimer’s disease pathology

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-06-03 DOI:10.1016/j.isci.2025.112817

Huifen Ma , Zhiyang Yu , Qiong Qiao , Wenpan Wang , Zhonghua Li , Pan Wang , Junying Song , Xiaowei Zhang , Yunfang Su , Yiran Sun , Zhishen Xie , Zhenqiang Zhang

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Abstract

There has been increasing interest in the connection between AD, gut microbiota, and metabolites. Kai-Xin-San (KXS) has been commonly employed in ancient and modern Chinese clinical trials for the treatment of dementia; however, whether the protective effect of KXS in AD is related to the gut microbiota remains elusive. APP/PS1 mice were used as the model of AD. 43 key metabolites influenced by KXS were screened using untargeted metabolomics. At the genus level, Clostridium_IV, Eubacterium, Acetatifactor, etc., were identified to be impacted by KXS using 16S rRNA sequencing. Additionally, we identified 9 distinct intestinal floras at the genus level that were correlated with 13 pivotal differential metabolites related to cognitive impairment. KXS also inhibited the neuroinflammation, mostly via regulating the key metabolites. A potential relationship between gut microbiota, metabolites, and neuroinflammation is suggested as a protective mechanism of KXS in AD. These findings provide support for further development of KXS.

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代谢组学和微生物学见解：开心散对阿尔茨海默病病理的影响

人们对阿尔茨海默病、肠道微生物群和代谢物之间的联系越来越感兴趣。开心散（KXS）在中国古今临床试验中常用，用于治疗痴呆；然而，KXS对AD的保护作用是否与肠道菌群有关尚不清楚。以APP/PS1小鼠为AD模型。利用非靶向代谢组学方法筛选了43种受KXS影响的关键代谢物。在属水平上，通过16S rRNA测序，鉴定出Clostridium_IV、Eubacterium、Acetatifactor等受KXS影响。此外，我们在属水平上鉴定了9种不同的肠道菌群，这些菌群与13种与认知障碍相关的关键差异代谢物相关。KXS对神经炎症也有抑制作用，主要通过调节关键代谢物。肠道菌群、代谢物和神经炎症之间的潜在关系被认为是KXS在AD中的保护机制。这些发现为KXS的进一步发展提供了支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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