Optimization of enzymatic saccharification of pretreated sugarcane bagasse for enhanced polyhydroxybutyrate (PHB) production by Bacillus megaterium PNCM 1890

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-06-13 DOI:10.1016/j.bcab.2025.103634

Ryan Christian R. Suplito , Princess J. Requiso , Catalino G. Alfafara , Fidel Rey P. Nayve Jr. , Jey-R S. Ventura

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引用次数: 0

Abstract

Polyhydroxyalkanoates (PHAs) are microbial polyesters that serve as biodegradable and bio-based alternatives to conventional plastics. Using renewable feedstocks, such as lignocellulosic biomass, offers a sustainable approach to PHA production while mitigating petrochemical dependence and plastic waste accumulation. In this study, enzymatic saccharification of sequentially pretreated sugarcane bagasse (SCB) was optimized to maximize hydrolysate yield for polyhydroxybutyrate (PHB) fermentation. Key parameters—solids loading, enzyme loading, and saccharification time—were evaluated for their impact on reducing sugar yield (RSY). A numeric factorial experiment confirmed their significant effects, and response surface methodology with a face-centered central composite design was employed. ANOVA results revealed the significance of the main and quadratic effects of these factors on RSY. Optimization identified ideal conditions as 4.94 % (w/v) solids loading, 102.98 filter paper units (FPU)/g dry biomass enzyme loading, and 70.44 h of saccharification, yielding 0.46 g reducing sugar/g dry biomass. Fermentation of the optimized hydrolysate achieved a maximum PHB concentration of 6.11 g/L after 20 h, with glucose consumption exceeding xylose. These findings provide a foundation for local bioplastic production using sugarcane bagasse.

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大芽孢杆菌PNCM 1890对预处理甘蔗渣酶解糖化提高聚羟基丁酸酯（PHB）产量的优化研究

聚羟基烷酸酯（PHAs）是一种微生物聚酯，可作为传统塑料的可生物降解和生物基替代品。使用可再生原料，如木质纤维素生物质，为PHA生产提供了一种可持续的方法，同时减少了对石化产品的依赖和塑料废物的积累。本研究优化了顺序预处理甘蔗渣（SCB）的酶解糖化，以最大限度地提高聚羟基丁酸酯（PHB）发酵的水解产物产量。研究了固体负荷、酶负荷和糖化时间等关键参数对还原糖产量的影响。数值因子实验证实了它们的显著效果，并采用面心中心复合设计的响应面法。方差分析结果显示，这些因素对RSY的主效应和二次效应显著。优化确定的理想条件为：固体负荷为4.94% (w/v)，干生物质酶负荷为102.98滤纸单位(FPU)/g，糖化时间为70.44 h，每g干生物质产0.46 g还原糖。优化后的水解液发酵20 h后PHB浓度达到6.11 g/L，葡萄糖消耗超过木糖。这些发现为当地利用甘蔗渣生产生物塑料提供了基础。

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.