A versatile ITO electrode platform for studying neuronal differentiation and migration under electrical stimulation

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X Pub Date : 2025-06-09 DOI:10.1016/j.biosx.2025.100637

Suthiwan Udomrat , Supeecha Kumkate , Permphan Dharmasaroja , Theeraporn Puntheeranurak , Tanakorn Osotchan

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引用次数: 0

Abstract

The electrical effects on cells, particularly neurons, are extensively studied to understand cellular functions. Various culture platforms have been developed to investigate cellular responses to electrical stimulation. In this study, planar indium tin oxide (ITO) interdigitated electrodes (IDEs) with conventional and circular designs were fabricated and utilized as both cell culture platforms and stimulation electrodes for experiments up to ten days. To enhance cell adhesion, fetal bovine serum (FBS) was applied to the electrode surface and then removed, facilitating cell culture. The SH-SY5Y cell line was cultured on the platform to assess adhesion and growth. Steady-state direct-current electric field stimulation (DCEFS) at 150 V/m or 300 V/m was applied at specific intervals. Cells exposed to 150 V/m showed significant morphological changes indicative of differentiation, while those at 300 V/m migrated away, reducing intact cell numbers. Notably, circular IDEs promoted extensive neurite outgrowth compared to conventional designs. These findings highlight the potential of electrode geometry to control cell differentiation and migration, offering a versatile platform for cellular studies and tissue engineering applications.

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研究电刺激下神经元分化和迁移的多功能ITO电极平台

对细胞，特别是神经元的电效应进行了广泛的研究，以了解细胞功能。已经开发了各种培养平台来研究细胞对电刺激的反应。在这项研究中，平面氧化铟锡（ITO）交叉电极（IDEs）具有传统和圆形设计，并被用作细胞培养平台和刺激电极，实验时间长达10天。为了增强细胞粘附，将胎牛血清（FBS）涂于电极表面，然后去除，促进细胞培养。在平台上培养SH-SY5Y细胞系，观察其粘附和生长情况。以特定的间隔施加150 V/m或300 V/m的稳态直流电场刺激（DCEFS）。暴露于150 V/m的细胞表现出明显的形态变化，表明分化，而暴露于300 V/m的细胞则迁移，减少了完整细胞的数量。值得注意的是，与传统设计相比，圆形ide促进了广泛的神经突生长。这些发现突出了电极几何结构控制细胞分化和迁移的潜力，为细胞研究和组织工程应用提供了一个通用的平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics: X Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

166

审稿时长

54 days

期刊介绍： Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.