Biocontrol potential of metabolites from Streptomyces sp., non-toxic Aspergillus fumigatus, and their co-cultures against tomato anthracnose

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2025-06-16 DOI:10.1016/j.bse.2025.105067

Tavga Sulaiman Rashid , Hayman Kakakhan Awla , Rouhallah Sharifi

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引用次数: 0

Abstract

The biological activity of Streptomyces sp. and non-toxic Aspergillus fumigatus, along with their metabolites in mono and co-culture, was tested against Colletotrichum acutatum. Additionally, the presence of volatile metabolites was observed using gas chromatography-mass spectrometry (GC-MS). The tomato plants were treated with both Streptomyces sp. and A. fumigatus separately and in combination for in vivo test. Both Streptomyces sp. and A. fumigatus exhibited antifungal activity against C. acutatum. In co-cultivation, metabolites demonstrated 100 % inhibition of the pathogenic fungus. According to GC-MS analysis, 35 compounds were identified from A. fumigatus, and 21 from Streptomyces sp. Co-cultivation of A. fumigatus and Streptomyces sp. induced the biosynthesis of 21 compounds. Most of volatile compounds present at high percentages in co-culture were not detected in the monoculture of each microorganism. The top volatiles in co-culture included acetic acid (25.58 %), furfural (9.89 %), Levulinic acid, methyl ester (7.22 %), dimethadione (6.50 %), benzene acetic acid (6.03 %), and levulinic acid (5.93 %). Significant improvements in shoot height, root length, shoot and root dry weight and the anthracnose disease reduction were observed mainly with the combination of Streptomyces sp. and A. fumigatus. These outcomes support the use of the co-cultivation technique as a viable approach for acquiring beneficial metabolites from microorganisms.

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链霉菌、烟曲霉及其共培养物代谢物对番茄炭疽病的生物防治潜力

研究了链霉菌和无毒烟曲霉及其代谢物在单株和共培养下对炭疽病菌的生物活性。此外，使用气相色谱-质谱（GC-MS）观察挥发性代谢物的存在。用烟曲霉和链霉菌分别和联合对番茄植株进行体内试验。链霉菌（Streptomyces sp.）和烟曲霉（A. fumigatus）对尖头葡萄球菌均有抑菌活性。在共同培养中，代谢物对病原菌的抑制作用达到100%。通过GC-MS分析，从烟曲霉中鉴定出35个化合物，从链霉菌中鉴定出21个化合物，其中烟曲霉与链霉菌共培养诱导了21个化合物的合成。大多数挥发性化合物在共培养中以高百分比存在，在每种微生物的单一培养中未检测到。共培养挥发物最高的挥发物为乙酸（25.58%）、糠醛（9.89%）、乙酰丙酸、甲酯（7.22%）、二甲二酮（6.50%）、苯乙酸（6.03%）、乙酰丙酸（5.93%）。在茎高、根长、茎干重和根干重等方面，烟曲霉和链霉菌联合施用对防治炭疽病效果显著。这些结果支持使用共培养技术作为从微生物中获取有益代谢物的可行方法。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.