Sample motion for structured illumination fluorescence microscopy.

IF 3.1 2区物理与天体物理 Q2 OPTICS

Optics letters Pub Date : 2025-06-15 DOI:10.1364/OL.560873

Ruiming Cao, Guanghan Meng, Laura Waller

引用次数: 0

Abstract

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single speckle illumination pattern and relying on inherent sample motion to encode the super-resolved information in multiple raw images. From a set of raw fluorescence images captured as the sample moves, we jointly estimate both the sample motion and the super-resolved image. We demonstrate the feasibility of the proposed method both in simulation and in experiment.

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结构照明荧光显微镜的样品运动。

结构照明显微镜（SIM）使用一组以不同照明模式捕获的图像来计算重建超出衍射极限的分辨率。在这里，我们提出了一种替代方法，使用单个散斑照明模式并依赖于固有的样本运动来编码多张原始图像中的超分辨率信息。从样本移动时捕获的一组原始荧光图像中，我们共同估计了样本运动和超分辨率图像。通过仿真和实验验证了该方法的可行性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Optics letters 物理-光学

CiteScore

6.60

自引率

8.30%

发文量

2275

审稿时长

1.7 months

期刊介绍： The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community. Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.