Polarimetric coherent anti-Stokes Raman scattering microscopy of collagen.

IF 3.1 2区物理与天体物理 Q2 OPTICS

Optics letters Pub Date : 2025-06-15 DOI:10.1364/OL.557999

Lingxiao Yang, Janet E Sorrells, Rishyashring R Iyer, Stephen A Boppart

引用次数: 0

Abstract

We demonstrate polarimetric coherent anti-Stokes Raman scattering (PCARS) microscopy for quantitative analysis of collagen orientation. Using polarization-modulated excitation and cross-polarized detection, we extract both chemical and orientational information from intact tissues. We introduce two complementary analyses: the CARS peak polarization ratio (PPR) and Fourier domain polarimetric analysis, providing a robust characterization of collagen organization. This method enables rapid, label-free imaging of a collagen structure with molecular specificity, offering what we believe to be new capabilities for studying collagen remodeling in biological processes.

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胶原蛋白的偏振相干抗斯托克斯拉曼散射显微镜。

我们展示了偏振相干抗斯托克斯拉曼散射（PCARS）显微镜用于胶原取向的定量分析。利用偏振调制激发和交叉极化检测，我们从完整的组织中提取化学和取向信息。我们介绍了两种互补分析：CARS峰极化比（PPR）和傅里叶域极化分析，提供了胶原组织的稳健表征。这种方法能够快速、无标记地成像具有分子特异性的胶原结构，为研究生物过程中的胶原重塑提供了我们认为的新能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Optics letters 物理-光学

CiteScore

6.60

自引率

8.30%

发文量

2275

审稿时长

1.7 months

期刊介绍： The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community. Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.