Fifth-harmonic and five-photon excitation fluorescence multiphoton microscopy.

IF 3.1 2区物理与天体物理 Q2 OPTICS

Optics letters Pub Date : 2025-06-15 DOI:10.1364/OL.566910

Joshua H Magnus, Lam T Nguyen, Elana G Alevy, Travis W Sawyer, Samuel D Crossley, Khanh Kieu

引用次数: 0

Abstract

We report the application of fifth-harmonic and five-photon excitation fluorescence multiphoton microscopy. This novel, to our knowledge, label-free imaging modality has been characterized with spectral and power dependence measurements. The advantages of fifth-harmonic and five-photon excitation fluorescence imaging are higher resolution and use of longer wavelength excitation sources, which may allow deeper penetration depth while still exciting near-UV to blue fluorophores. This imaging modality may find interesting applications in biological research, materials characterization, geologic studies, and semiconductor manufacturing.

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五次谐波和五光子激发荧光多光子显微镜。

我们报道了五次谐波和五光子激发荧光多光子显微镜的应用。据我们所知，这种新颖的无标签成像方式具有光谱和功率依赖测量的特点。五次谐波和五光子激发荧光成像的优点是更高的分辨率和使用更长的波长激发源，这可能允许更深的穿透深度，同时仍然激发近紫外到蓝色荧光团。这种成像模式可能会在生物研究、材料表征、地质研究和半导体制造中找到有趣的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Optics letters 物理-光学

CiteScore

6.60

自引率

8.30%

发文量

2275

审稿时长

1.7 months

期刊介绍： The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community. Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.