Antitumor Effect of Isoliquiritigenin via Mitochondrial Dysfunction and Oxidative Stress in Cholangiocarcinoma Cells.

IF 2 3区 农林科学 Q4 CHEMISTRY, MEDICINAL
Journal of medicinal food Pub Date : 2025-09-01 Epub Date: 2025-06-13 DOI:10.1089/jmf.2024.k.0280
Sang Hyeok Bae, Hwayoung Na, Dohee Ahn, Hong Kyu Lee, Kyung-Chul Choi
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引用次数: 0

Abstract

Isoliquiritigenin (ISL), a bioactive phytochemical derived from the root of Glycyrrhiza uralensis, is known to exert anticancer effects by modulating cancer cell functions, including proliferation, metastasis, and autophagy. However, the anticancer effects of ISL on cholangiocarcinoma (CCA) are limited. In this study, we investigated the anticancer effects of ISL on CCA cells (SNU-478 and HuCCT-1). In SNU-478 cells, ISL decreased the expression of glucose-regulated protein 78 (GRP78). ISL was also found to significantly reduce cell viability and colony area of both SNU-478 and HuCCT-1 cells. Furthermore, ISL treatment significantly increased the population of apoptotic cells, as observed through Annexin V/propidium iodide (PI) staining. PI staining showed that the ISL treatment induced significant cell cycle arrest compared to the control group, indicating that ISL-induced decreases in cell viability are associated with cell cycle arrest and apoptosis. ISL treatment also resulted in a significant decline in the mitochondrial membrane potential when observed through JC-10 staining. Mitochondrial superoxide staining revealed that ISL significantly upregulated the generation of mitochondrial reactive oxygen species (ROS). Acridine orange staining indicated that the ISL treatment upregulated lysosomal membrane permeability, which could be related to autophagy. These results suggest that ISL affects the proliferation and apoptosis of CCA cells, which might be related to its regulatory role in various cellular functions, including mitochondrial dysfunction, ROS balance, and autophagy. This study provides empirical evidence that ISL could be a potential candidate for CCA therapy.

异尿酸原素通过胆管癌细胞线粒体功能障碍和氧化应激的抗肿瘤作用。
异甘草素(ISL)是一种从甘草根中提取的生物活性植物化学物质,已知通过调节癌细胞的功能,包括增殖、转移和自噬,发挥抗癌作用。然而,ISL对胆管癌(CCA)的抗癌作用有限。在本研究中,我们研究了ISL对CCA细胞(SNU-478和HuCCT-1)的抗癌作用。在SNU-478细胞中,ISL降低葡萄糖调节蛋白78 (GRP78)的表达。ISL还可显著降低SNU-478和HuCCT-1细胞的细胞活力和集落面积。此外,通过膜联蛋白V/碘化丙啶(PI)染色观察到,ISL处理显著增加了凋亡细胞的数量。PI染色显示,与对照组相比,ISL处理诱导了显著的细胞周期阻滞,表明ISL诱导的细胞活力下降与细胞周期阻滞和凋亡有关。通过JC-10染色观察,ISL处理也导致线粒体膜电位显著下降。线粒体超氧化物染色显示,ISL显著上调线粒体活性氧(ROS)的产生。吖啶橙染色表明,ISL处理上调了溶酶体膜通透性,这可能与自噬有关。这些结果表明,ISL影响CCA细胞的增殖和凋亡,这可能与其在线粒体功能障碍、ROS平衡和自噬等多种细胞功能中的调节作用有关。本研究提供的经验证据表明,ISL可能是CCA治疗的潜在候选者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of medicinal food
Journal of medicinal food 医学-食品科技
CiteScore
4.50
自引率
0.00%
发文量
154
审稿时长
4.5 months
期刊介绍: Journal of Medicinal Food is the only peer-reviewed journal focusing exclusively on the medicinal value and biomedical effects of food materials. International in scope, the Journal advances the knowledge of the development of new food products and dietary supplements targeted at promoting health and the prevention and treatment of disease.
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