Different responses of the TGF-β superfamily genes in the warm-water noble scallop Chlamys nobilis to acute and chronic low temperature stress

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-06-13 DOI:10.1016/j.aquaculture.2025.742852

Shitong Liu , Dewei Cheng , Meizhen Chen , Huiqi Li , Naiqian Zhao , Yaoting Liu , Beiyu Yan , Weili Wang , Hongkuan Zhang , Huaiping Zheng

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引用次数: 0

Abstract

The Transforming Growth Factor-β (TGF-β) superfamily is comprised of proteins with diverse biological activities, playing a crucial role in immunity, nevertheless few studies have explored their functions in aquatic animals. In this study, 560 TGF-β superfamily genes were first identified through whole-genome analysis from 33 bivalve species, categorized into 10 clusters. Among these, 13 CnTGFB genes were identified in the noble scallop Chlamys nobilis, an important cultured warm-water bivalve in China, were grouped into 9 clusters. The analysis of tissue-specific expression revealed that compared with the testis, the mantle and the adductor muscle, the expression level of the CnTGFB gene was higher in the ovary, the gills, the kidneys and the blood. Under the acute low temperature stress (temperature reduced from 23 °C to 9 °C within 24 h), the expression levels of CnTGFB-02, CnTGFB-07, and CnTGFB-08 were significantly increased, while CnTGFB-01a, CnTGFB-01b, CnTGFB-03a, and CnTGFB-05b were significantly decreased. Under the chronic low temperature stress (gradual reduction from 23 °C to 9 °C over 30 days) induced distinct expression patterns: CnTGFB-01a, CnTGFB-02, CnTGFB-03a, CnTGFB-05a, CnTGFB-07, CnTGFB-08 and CnTGFB-10 showed a significant up-regulation when the temperature declined at 10 °C (P < 0.05), whereas CnTGFB-01b, CnTGFB-04, CnTGFB-05b and CnTGFB-09 exhibited significantly down-regulation when the temperature declined at 17 °C (P < 0.05). Moreover, only CnTGFB-05b had significantly higher expression under acute low temperature stress, while 9 genes were more responsive to chronic stress at 9 °C (P < 0.05), and CnTGFB-02 had no significantly difference expression at 9 °C (P > 0.05). These results indicated that TGF-β genes play important roles under low temperature stress in the noble scallop, which are also useful for breeding low temperature tolerant varieties in warm-water bivalves.

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温水贵族扇贝TGF-β超家族基因对急慢性低温胁迫的不同响应

转化生长因子-β (TGF-β)超家族是由具有多种生物活性的蛋白质组成的，在免疫中起着至关重要的作用，但很少有研究探讨其在水生动物中的功能。本研究通过对33种双壳类动物的全基因组分析，首次鉴定出560个TGF-β超家族基因，并将其分为10个簇。其中，13个CnTGFB基因在中国重要的养殖暖水双壳类栉孔扇贝（Chlamys nobilis）中被鉴定出来，并分为9个簇。组织特异性表达分析显示，与睾丸、套膜和内收肌相比，CnTGFB基因在卵巢、鳃、肾脏和血液中的表达水平较高。在急性低温胁迫下（24 h内温度从23℃降至9℃），CnTGFB-02、CnTGFB-07和CnTGFB-08的表达量显著升高，而CnTGFB-01a、CnTGFB-01b、CnTGFB-03a和CnTGFB-05b的表达量显著降低。慢性低温胁迫（30 d内从23℃逐渐降低到9℃）诱导了不同的表达模式：CnTGFB-01a、CnTGFB-02、CnTGFB-03a、CnTGFB-05a、CnTGFB-07、CnTGFB-08和CnTGFB-10在温度降至10℃时显著上调(P <；0.05)，而CnTGFB-01b、CnTGFB-04、CnTGFB-05b和CnTGFB-09在17℃温度下降时表现出显著的下调(P <；0.05)。此外，只有CnTGFB-05b在急性低温胁迫下的表达量显著增加，而9个基因对9℃慢性应激的反应更强(P <；0.05)，而CnTGFB-02在9°C时的表达无显著差异(P >；0.05)。上述结果表明，TGF-β基因在贝类低温胁迫下发挥了重要作用，为培育耐低温双壳类动物品种提供了理论依据。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.