Study of Glutathione S-Transferase, Its Isoenzyme (Pi) and Tumor Marker (CA15-3, HE4) in Breast Carcinoma Patients (Before and After Treatment).

Abstract

Breast cancer is a prevalent malignancy among women worldwide, with prognosis and survival heavily influenced by early diagnosis and effective treatment. This study evaluates the diagnostic and prognostic significance of glutathione S-transferase (GST), its isoenzyme GST-Pi, and tumor markers CA15-3 and HE4 in breast carcinoma patients before and after treatment. It also explores the correlation of these biomarkers with liver enzymes (SGOT, SGPT, LDH, ALP), commonly associated with hepatic metastasis. A quasi-experimental design will include 311 participants from the Vidarbha region, clinically diagnosed with breast carcinoma. Blood samples will be analyzed using enzyme-linked immunosorbent assay (ELISA) and automated biochemical analyzers to estimate levels of GST, GST-Pi, CA15-3, HE4, and liver enzymes. The study aims to identify reliable biomarkers for monitoring therapeutic efficacy, assessing prognosis, and predicting recurrence in breast carcinoma. This research may contribute to developing cost-effective diagnostic approaches, particularly beneficial in resource-limited healthcare settings.