Plant-Based Potential in Diabetes Management: In Vitro Antioxidant, Wound-Healing, and Enzyme Inhibitory Activities of Southern Algarve Species.

IF 4.2 2区化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules Pub Date : 2025-06-01 DOI:10.3390/molecules30112432

Isabel S Carvalho, Cláudia Viegas, Marta Markiewicz, Agnieszka Galanty, Paweł Paśko, Lejsa Jakupović, Marijana Zovko Končić

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Abstract

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by impaired glucose regulation. This study evaluated the antioxidant and antidiabetic potential of aqueous extracts from four plant species from the southern Algarve: Aristolochia baetica, Chelidonium majus, Dittrichia viscosa, and Lavandula viridis, using non-cellular in vitro assays. HPLC/PDA was used to identify active compounds. Antioxidant activity was assessed by using TAA, FRAP, RP, and DPPH assays; antidiabetic potential through α-glucosidase and α-amylase inhibition; and wound healing relevance through elastase, collagenase, and lipoxygenase inhibition. D. viscosa showed the highest antioxidant activity (FRAP: 1132.99 ± 19.54 mg TE/g dw; DPPH IC₅₀ = 25.85 ± 0.75 μg/mL) and total phenolic/flavonoid content, with a diverse profile including caffeic and chlorogenic acids, isoquercetin, and quercetin. It also exhibited potent α-glucosidase inhibition (IC₅₀ = 0.61 ± 0.06 mg/mL), outperforming acarbose. L. viridis had the highest total phenolic content (39.04 mg/g), while A. baetica demonstrated the strongest anti-elastase, anti-collagenase, and lipoxygenase activity, suggesting wound-healing potential. C. majus showed the weakest effects. A strong correlation was observed between phenolic content and antioxidant/antidiabetic activity. These findings support further in vivo studies on D. viscosa and A. baetica for potential use in T2DM management and diabetic wound healing.

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植物在糖尿病管理中的潜力：体外抗氧化，伤口愈合和酶抑制活性的南阿尔加维物种。

2型糖尿病（T2DM）是一种以葡萄糖调节功能受损为特征的慢性代谢紊乱。本研究通过非细胞体外实验，评估了阿尔加维南部四种植物的水提取物的抗氧化和抗糖尿病潜力：马兜铃、大毛Chelidonium majus、粘毛滴虫和薰衣草。采用高效液相色谱法/PDA对活性成分进行鉴定。采用TAA、FRAP、RP和DPPH测定抗氧化活性；α-葡萄糖苷酶和α-淀粉酶抑制的降糖潜力并通过弹性酶、胶原酶和脂氧合酶抑制伤口愈合。粘胶多糖抗氧化活性最高（FRAP: 1132.99±19.54 mg TE/g dw）；DPPH IC50 = 25.85±0.75 μg/mL)和总酚/类黄酮含量，包括咖啡酸和绿原酸、异槲皮素和槲皮素。对α-葡萄糖苷酶有较强的抑制作用（IC50 = 0.61±0.06 mg/mL），优于阿卡波糖。绿脓杆菌总酚含量最高（39.04 mg/g），而白脓杆菌抗弹性酶、抗胶原酶和脂氧合酶活性最强，表明其具有创面愈合潜力。马氏菌的效果最弱。酚含量与抗氧化/抗糖尿病活性之间存在很强的相关性。这些发现支持了粘胶芽孢杆菌和贝氏芽孢杆菌在T2DM治疗和糖尿病伤口愈合中的潜在应用的进一步体内研究。

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来源期刊

Molecules 化学-有机化学

CiteScore

7.40

自引率

8.70%

发文量

7524

审稿时长

1.4 months

期刊介绍： Molecules (ISSN 1420-3049, CODEN: MOLEFW) is an open access journal of synthetic organic chemistry and natural product chemistry. All articles are peer-reviewed and published continously upon acceptance. Molecules is published by MDPI, Basel, Switzerland. Our aim is to encourage chemists to publish as much as possible their experimental detail, particularly synthetic procedures and characterization information. There is no restriction on the length of the experimental section. In addition, availability of compound samples is published and considered as important information. Authors are encouraged to register or deposit their chemical samples through the non-profit international organization Molecular Diversity Preservation International (MDPI). Molecules has been launched in 1996 to preserve and exploit molecular diversity of both, chemical information and chemical substances.