Development of Xanthyletin-Loaded Nanoparticles for the Control of Leucoagaricus gongylophorus.

IF 4.2 2区化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules Pub Date : 2025-06-05 DOI:10.3390/molecules30112469

Cristiane de Melo Cazal, Moacir Rossi Forim, Ana Paula Terezan, Andreia Pereira Matos, Gracielle Oliveira Sabbag Cunha, Maria Fátima das Graças Fernandes da Silva, Paulo Cezar Vieira, Fernando Carlos Pagnocca, João Batista Fernandes

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Abstract

This study describes the development, characterization and in vitro evaluation of poly(ε-caprolactone) (PCL) nanoparticles loaded with xanthyletin for the control of Atta sexdens rubropilosa through the inhibition of its symbiotic fungus Leucoagaricus gongylophorus. Nanoparticles were prepared via interfacial polymer deposition, with formulation NC5 selected based on optimal physicochemical properties. NC5 exhibited an encapsulation efficiency of 98.0%, average particle size of 304 nm and zeta potential of -29.3 mV. Scanning electron microscopy confirmed spherical morphology and the absence of crystalline residues. The formulation remained physically stable for four months at 4 °C. In vitro release showed biphasic behavior, with an initial burst followed by sustained release. Under UV exposure, NC5 enhanced xanthyletin photostability by 15.4-fold compared to the free compound. Fungicidal assays revealed 76% inhibition of fungal growth with NC5, compared to 85% with free xanthyletin. These results support the potential application of xanthyletin-loaded PCL nanoparticles as a stable and controlled delivery system for the biological control of leaf-cutting ants by targeting their fungal mutualist. Further in vivo studies are recommended to assess efficacy under field conditions.

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载黄叶素纳米颗粒防治白带松茸的研究。

本文研究了载黄素的聚（ε-己内酯）（PCL）纳米颗粒的制备、表征和体外评价，该纳米颗粒通过抑制其共生真菌白松茸（Leucoagaricus gongylophorus）来控制红叶白松茸（Atta sexdens rubbropilosa）。采用界面聚合物沉积法制备纳米颗粒，并根据最佳的理化性能选择配方NC5。NC5包封效率为98.0%，平均粒径为304 nm， zeta电位为-29.3 mV。扫描电子显微镜证实了球体形态和无结晶残留物。该配方在4°C下保持物理稳定4个月。体外释放表现为双相行为，先是爆发，然后是持续释放。在紫外线照射下，NC5使黄原素的光稳定性提高了15.4倍。杀真菌实验显示，NC5对真菌生长的抑制率为76%，而游离黄原素对真菌生长的抑制率为85%。这些结果支持了载黄叶素的PCL纳米颗粒作为一种稳定可控的递送系统的潜在应用，该系统可以通过靶向切叶蚁的真菌共生体来生物控制切叶蚁。建议进一步的体内研究来评估野外条件下的疗效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecules 化学-有机化学

CiteScore

7.40

自引率

8.70%

发文量

7524

审稿时长

1.4 months

期刊介绍： Molecules (ISSN 1420-3049, CODEN: MOLEFW) is an open access journal of synthetic organic chemistry and natural product chemistry. All articles are peer-reviewed and published continously upon acceptance. Molecules is published by MDPI, Basel, Switzerland. Our aim is to encourage chemists to publish as much as possible their experimental detail, particularly synthetic procedures and characterization information. There is no restriction on the length of the experimental section. In addition, availability of compound samples is published and considered as important information. Authors are encouraged to register or deposit their chemical samples through the non-profit international organization Molecular Diversity Preservation International (MDPI). Molecules has been launched in 1996 to preserve and exploit molecular diversity of both, chemical information and chemical substances.