Long-term analysis of Vibrio vulnificus and Vibrio parahaemolyticus virulence factors and their environmental associations in the Chesapeake Bay, Maryland, US.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2025-07-01 DOI:10.1093/jambio/lxaf145

Michele E Morgado, Kyle D Brumfield, Suhana Chattopadhyay, Leena Malayil, Anwar Huq, Rita R Colwell, Amy R Sapkota

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引用次数: 0

Abstract

Aims: The present study analyzed long-term trends in Vibrio spp. virulence factors associated with pathogenicity in Chesapeake Bay waters (in Maryland) across two 3-year sampling periods (2009-2012 and 2019-2022).

Methods and results: Vibrio parahaemolyticus (n = 1476) and V. vulnificus (n = 806) isolates were recovered from 13 sampling stations and tested for species-specific and virulence markers. Across both sampling periods, detection of V. parahaemolyticus tdh+/trh+ isolates was similar and more frequently observed in the spring and fall, and the upper sampling stations. However, significant differences were observed in the detection of V. vulnificus pilA+ and rtxA+ isolates, with a higher percentage of pilA+ in 2019-2022 and rtxA+ in 2009-2012. VcgC+ isolates were most frequently detected during the fall (n = 24%) in 2019-2022. Interestingly, a greater prevalence of V. vulnificus isolates harboring virulence markers was observed in the mid and lower stations.

Conclusions: Vibrio vulnificus isolates displayed greater changes in virulence marker frequency across sampling periods compared to V. parahaemolyticus and these markers were detectable throughout the year.

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美国马里兰州切萨皮克湾创伤弧菌和副溶血性弧菌毒力因子的长期分析及其环境关联

目的：本研究分析了马里兰州切萨皮克湾水域（2009-2012年和2019-2022年）两个三年采样期与致病性相关的弧菌毒力因子的长期趋势。方法与结果：从13个采样点回收副溶血性弧菌（1476株）和创伤性弧菌（806株），并进行了种特异性和毒力标记检测。在两个采样期间，副溶血性弧菌tdh+/trh+分离株的检测相似，并且在春季和秋季以及上层采样站更频繁地观察到。然而，创伤弧菌pilA+和rtxA+分离株的检出率存在显著差异，2019-2022年pilA+检出率较高，2009-2012年rtxA+检出率较高。2019-2022年，VcgC+分离株最常见于秋季（n=24%）。有趣的是，在中低站观察到携带毒力标记的创伤弧菌分离株的更大流行。结论：与副溶血性弧菌相比，创伤弧菌分离株在不同采样期间的毒力标记频率变化更大，这些标记在全年均可检测到。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.