Evaluation of an internet of things device for isothermal molecular detection.

IF 5.4 2区医学 Q1 INFECTIOUS DISEASES

Infection Pub Date : 2025-06-13 DOI:10.1007/s15010-025-02581-1

Manfred Weidmann, Jesus Bueno Alvarez, Steffen Zinn, Ruslan Ibragimov, Mohammed Hasani, Elena Graf, Aiko Weber, Frida Arrey, Marcelina Marta Szczygiel, Zuzanna Kowalewska, Rea Maja Kobialka, Arianna Ceruti, Ahmed Abd El Wahed, Michael Diebold, Christian Wahnes

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引用次数: 0

Abstract

We describe a low-cost up scalable molecular test platform providing accessibility to frequent testing - the prerequisite to control infectious epidemics. The device uses the Multi-Spectral Digital Sensor AS7341 in a small fluorescence spectrometer which communicates with a Smartphone App via Bluetooth, which passes on the raw data to outsourced computation in the cloud via the internet. This very stable setup allows easy to use on site testing of individual samples and anonymised data analysis while providing the basis for direct result transmission of a rapid isothermal molecular diagnostic test result not only to the user but also to public health bodies. This internet of things (IoT) platform allows to collect real molecular test data from users with usability and industrial up scalability at the heart of the design. The device characteristics are described in detail and the performance for a rapid SARS-CoV-2 Recombinase Polymerase Amplification assay achieved 98.6% sensitivity and 98% specificity testing 148 positive and 501 negative samples.

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物联网等温分子检测装置的评价。

我们描述了一个低成本的可扩展的分子测试平台，提供了频繁测试的可访问性-控制传染病的先决条件。该设备在一个小型荧光光谱仪中使用多光谱数字传感器AS7341，该光谱仪通过蓝牙与智能手机应用程序通信，智能手机应用程序通过互联网将原始数据传递给云中的外包计算。这种非常稳定的设置可以方便地在现场测试单个样品和匿名数据分析，同时为快速等温分子诊断测试结果的直接传递提供基础，不仅向用户，而且向公共卫生机构。这个物联网（IoT）平台允许从用户那里收集真实的分子测试数据，其可用性和工业可扩展性是设计的核心。详细描述了该装置的特点，对148份阳性和501份阴性样品进行了SARS-CoV-2重组酶聚合酶扩增快速检测，灵敏度达到98.6%，特异性达到98%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection 医学-传染病学

CiteScore

12.50

自引率

1.30%

发文量

224

审稿时长

6-12 weeks

期刊介绍： Infection is a journal dedicated to serving as a global forum for the presentation and discussion of clinically relevant information on infectious diseases. Its primary goal is to engage readers and contributors from various regions around the world in the exchange of knowledge about the etiology, pathogenesis, diagnosis, and treatment of infectious diseases, both in outpatient and inpatient settings. The journal covers a wide range of topics, including: Etiology: The study of the causes of infectious diseases. Pathogenesis: The process by which an infectious agent causes disease. Diagnosis: The methods and techniques used to identify infectious diseases. Treatment: The medical interventions and strategies employed to treat infectious diseases. Public Health: Issues of local, regional, or international significance related to infectious diseases, including prevention, control, and management strategies. Hospital Epidemiology: The study of the spread of infectious diseases within healthcare settings and the measures to prevent nosocomial infections. In addition to these, Infection also includes a specialized "Images" section, which focuses on high-quality visual content, such as images, photographs, and microscopic slides, accompanied by brief abstracts. This section is designed to highlight the clinical and diagnostic value of visual aids in the field of infectious diseases, as many conditions present with characteristic clinical signs that can be diagnosed through inspection, and imaging and microscopy are crucial for accurate diagnosis. The journal's comprehensive approach ensures that it remains a valuable resource for healthcare professionals and researchers in the field of infectious diseases.