Comparative Transcriptomic Analysis Between High- and Low-Growth-Rate Meat-Type Rabbits Reveals Key Pathways Associated with Muscle Development.

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animals Pub Date : 2025-05-29 DOI:10.3390/ani15111585

Chao Yang, Lingxi Zhu, Li Tang, Xiangyu Zhang, Min Lei, Xiaohong Xie, Cuixia Zhang, Dingsheng Yuan, Congyan Li, Ming Zhang

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Abstract

Rabbit meat constitutes a high-protein, low-fat nutritional resource demonstrating rising consumption, particularly within the Asia-Pacific region. Consequently, muscle growth and developmental pattern in meat rabbits represent critical economic considerations. To elucidate the primary signaling pathways governing muscle development, we first performed comparative body weight analyses between two rabbit breeds exhibiting divergent growth rates: the fast-growing Checkered Giant (Ju) and slow-growing Sichuan Ma rabbit. Subsequent, post-natal qualities of thigh and longissimus dorsi muscle fiber were quantified across three developmental phases (28, 56, and 84 days post-natal). The results showed the body weight of Ju rabbit was significantly higher than that of Ma rabbit beyond 3 weeks post-natal (p < 0.05), while Ma rabbit exhibited larger muscle fiber areas in both tissues at 56 days (p < 0.05). The transcriptome analysis showed that 284 and 305 differentially expressed genes (DEGs) (|log2FC| > 1, padj < 0.05) were identified in thigh muscle and longissimus dorsi muscle, respectively. GO (Gene Ontology) analysis of DEGs indicated DEGs in the thigh muscle were enriched in these terms related to biological processes of muscle cell migration and smooth muscle cell migration, cellular components of sarcomere, myofibril, and actin filament bundle, while DEGs in longissimus dorsi muscle were enriched in these terms associated with biological processes of muscle cell migration, smooth muscle cell migration and muscle structure development, cellular component of actin cytoskeleton, contractile fiber, myofibril, myosin complex and molecular function of actin filament binding. Integrated GO, KEGG and PPI analyses of co-expressive DEGs implicated the HIF-1 signaling pathway and Glycolysis/Gluconeogenesis in muscular development. Different expression of energy metabolism hub-genes might be the primary reason for interbreed muscle developmental disparities.

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高和低生长速度肉兔的转录组学比较分析揭示了与肌肉发育相关的关键途径。

兔肉是一种高蛋白、低脂肪的营养资源，消费量不断上升，尤其是在亚太地区。因此，肉兔的肌肉生长和发育模式是重要的经济考虑因素。为了阐明控制肌肉发育的主要信号通路，我们首先对两种生长速度不同的兔子品种进行了体重比较分析：快速生长的巨格兔（Ju）和生长缓慢的四川麻兔。随后，在三个发育阶段（出生后28、56和84天）对大腿和背最长肌肌纤维的产后质量进行量化。结果表明：出生后3周以上，巨兔体重显著高于马家兔（p < 0.05）， 56 d时，马家兔两种组织肌纤维面积均较大（p < 0.05）。转录组分析显示，在大腿肌和背最长肌中分别鉴定出284个和305个差异表达基因（DEGs）（|log2FC| > 1, padj < 0.05）。GO （Gene Ontology）分析结果表明，大腿肌中的DEGs富集了与肌肉细胞迁移、平滑肌细胞迁移、肌节、肌原纤维、肌动蛋白丝束等生物过程相关的术语，背最长肌中的DEGs富集了与肌肉细胞迁移、平滑肌细胞迁移、肌肉结构发育、肌动蛋白细胞骨架等生物过程相关的术语。收缩纤维、肌原纤维、肌球蛋白复合物与肌动蛋白丝结合的分子功能。对共表达deg的综合GO、KEGG和PPI分析表明，HIF-1信号通路和肌肉发育中的糖酵解/糖异生。能量代谢中心基因的不同表达可能是杂种肌肉发育差异的主要原因。

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来源期刊

Animals Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

4.90

自引率

16.70%

发文量

3015

审稿时长

20.52 days

期刊介绍： Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).