Simple Diaminonucleoside-Mediated Nonenzymatic Ligation of Oligonucleotides.

IF 4 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Michael J Capperauld, Krish Kiran Valluru, Jagandeep S Saraya, Evan Zakaria, Connor E Wong, Derek K O'Flaherty
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引用次数: 0

Abstract

Oligonucleotide-templated chemical ligation is a powerful and robust technique to covalently join two or more oligonucleotides. Chemical handles, such as amino-modifiers, can be incorporated to enhance ligation efficiency. However, their incorporation is typically laborious, expensive, and time-consuming. Utilizing 3',5'-diamino-3',5'-dideoxythymidine (or 3',5'-diamino-2',3',5'-trideoxy-5-methylcytidine) and a water-soluble condensing reagent, we demonstrate a simple and cost-effective methodology for chemically ligating two oligonucleotides containing opposing monophosphate groups in a template-directed manner. Through reaction optimization, product formation reached >85% within 4 h in DNA, RNA-like, and certain chimeric-based systems. Our methodology will find applications in chemical biology and biotechnology.

简单二氨基核苷介导的寡核苷酸非酶连接。
寡核苷酸模板化学连接是一种强大而稳健的技术,以共价连接两个或多个寡核苷酸。化学处理,如氨基改性剂,可以纳入,以提高结扎效率。然而,它们的合并通常是费力的、昂贵的和耗时的。利用3',5'-二氨基-3',5'-二脱氧胸苷(或3',5'-二氨基-2',3',5'-三脱氧-5-甲基胞苷)和一种水溶性浓缩试剂,我们展示了一种简单而经济有效的方法,以模板导向的方式化学连接两个含有相反单磷酸基团的寡核苷酸。通过反应优化,在DNA、rna样和某些嵌合物为基础的体系中,4 h内的产物生成率达到了85%。我们的方法将在化学生物学和生物技术中得到应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Bioconjugate Chemistry
Bioconjugate Chemistry 生物-化学综合
CiteScore
9.00
自引率
2.10%
发文量
236
审稿时长
1.4 months
期刊介绍: Bioconjugate Chemistry invites original contributions on all research at the interface between man-made and biological materials. The mission of the journal is to communicate to advances in fields including therapeutic delivery, imaging, bionanotechnology, and synthetic biology. Bioconjugate Chemistry is intended to provide a forum for presentation of research relevant to all aspects of bioconjugates, including the preparation, properties and applications of biomolecular conjugates.
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