Myricetin, as a potential new antiviral agent against Rana grylio ranavirus (RGRV)

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-06-12 DOI:10.1016/j.fsi.2025.110492

Kun Peng , Silu Tian , Le Xu , Yu Yuan , Mingqi Ai , Qibin Jiang , Keyu Zhou , Ping Ouyang , Xiaoli Huang , Defang Chen , Yi Geng

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Abstract

Ranaviruses are extremely lethal viruses that pose a significant threat to aquaculture. Therefore, developing therapeutic strategies is essential for controlling these viruses. Utilizing herbal bioactive ingredients for the prevention and management of aquatic diseases shows great promise. In this study, we evaluated the antiviral activity of four flavonoids against a Ranavirus (RGRV). The results indicated that myricetin (MYR), luteolin (LUT), epigallocatechin gallate (EGCG), and rutin (RUT) exhibited anti-RGRV activity in vitro. Among these, MYR demonstrated the most potent antiviral activity, which was found to be dose-dependent. Subsequently, we discovered that MYR inhibited all stages of RGRV infection, particularly the early stage of viral replication, by down-regulating the expression of immediate-early genes and delayed-early genes. Furthermore, MYR enhanced host cell immunity and reduced inflammation by up-regulating the JAK-STAT signaling pathway and down-regulating the NF-κB signaling pathway. The in vivo challenge assay showed that MYR significantly reduced the mortality rate of the pig frog (Rana grylio) infected with RGRV. In summary, MYR demonstrated excellent anti-RGRV activity and held considerable promise for development in aquaculture. Our investigation provided the basis for promoting the development of anti-Ranaviruses agents.

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杨梅素作为一种潜在的新型抗病毒药物的研究

ranavirus是一种极其致命的病毒，对水产养殖构成重大威胁。因此，制定治疗策略对于控制这些病毒至关重要。利用草药生物活性成分预防和管理水生疾病具有广阔的前景。在这项研究中，我们评估了四种黄酮类化合物对一种Ranavirus （RGRV）的抗病毒活性。结果表明，杨梅素（MYR）、木犀草素（LUT）、没食子儿茶素没食子酸酯（EGCG）和芦丁（RUT）在体外具有抗rgrv的活性。其中，MYR显示出最有效的抗病毒活性，发现其具有剂量依赖性。随后，我们发现MYR通过下调立即早期基因和延迟早期基因的表达，抑制了RGRV感染的所有阶段，特别是病毒复制的早期阶段。此外，MYR通过上调JAK-STAT信号通路，下调NF-κB信号通路，增强宿主细胞免疫，减少炎症。体内攻毒实验表明，MYR显著降低了感染RGRV的猪蛙（Rana grylio）的死亡率。综上所述，最高产量研究显示出出色的抗rgrv活性，在水产养殖中具有相当大的发展前景。本研究为促进抗ranvirus药物的开发提供了依据。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.