The influence of echocardiography as a stressful manipulation on substance P, cortisol, and behavior in calves – A pilot study

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-06-12 DOI:10.1016/j.rvsc.2025.105751

H. Kerber, M. Feist, Y. Zablotski, G. Knubben-Schweizer, T. Tschoner

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引用次数: 0

Abstract

The aim of this pilot study was to investigate the effects of a stressful stimulus on plasma substance P concentrations (PSPC), salivary substance P (SSPC), plasma cortisol (PCC), and glucose concentrations, behavioral parameters, and milk intake in calves. Animals in STRESS (n = 12) were exposed to restrainment in a crush and ultrasonographic examination. Animals in CON (n = 12) remained in their igloo. Sampling was done prior (60 min before, 0 min), during (5 to 30 min), and after stimulation (35 min to 2 h). Activity and milk intake were assessed over 24 h; behavior during the stimulus and at sampling times. PSPC and PCC did not differ between groups. In STRESS, PSPC were significantly lower at 5 and 20 min compared with 60 min before. SSPC were below the sensitivity of the ELISA in 34 % of samples. SSPC were significantly higher in CON compared with STRESS at 0, 5, 10, 20, and 30 min, and at 2 h. In STRESS, SSPC were significantly lower at 30 min compared with 60 min before. There was no correlation between PSPC and SSPC. PCC were significantly higher at 20 min compared with 60 min before in STRESS, and significantly lower at 10 and 25 min compared with 60 min before in CON. Glucose concentrations were significantly lower in STRESS compared with CON at 5 and 10 min and differed significantly within groups. “Ear movement”, “limb movement”, and “urination” was significantly higher in STRESS compared with CON. Milk intake and activity did not differ between groups.

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超声心动图作为应激操作对小腿P物质、皮质醇和行为的影响-一项初步研究

本初步研究旨在探讨应激刺激对犊牛血浆P物质浓度（PSPC）、唾液P物质浓度（SSPC）、血浆皮质醇（PCC）和葡萄糖浓度、行为参数和奶摄入量的影响。应激动物（n = 12）在挤压和超声检查中暴露于约束。对照组（n = 12）的动物呆在它们的冰屋里。分别在刺激前（60分钟、0分钟）、刺激中（5 ~ 30分钟）和刺激后（35分钟~ 2小时）进行采样。在24小时内评估活动和牛奶摄入量；在刺激时和采样时的行为。PSPC和PCC组间差异无统计学意义。在应激状态下，PSPC在5和20 min较60 min前显著降低。SSPC在34%的样品中低于ELISA的灵敏度。在0、5、10、20、30 min和2 h时，CON组的SSPC显著高于应激组；在应激组，30 min时SSPC显著低于前60 min。PSPC与SSPC无相关性。应激组PCC在20 min显著高于前60 min，对照组在10、25 min显著低于前60 min。应激组葡萄糖浓度在5、10 min显著低于前60 min，组内差异显著。与对照组相比，应激组的“耳动”、“肢体运动”和“排尿”显著增加。各组之间的牛奶摄入量和活动没有差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.