Biological characteristics of high-searching individuals of the Heterorhabditis bacteriophora HBH hybrid strain for use in biocontrol

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-06-11 DOI:10.1016/j.cropro.2025.107313

Alperen Kaan Bütüner, İsmail Alper Susurluk

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Abstract

Entomopathogenic nematodes (EPNs) are widely used in biological control to manage insect pests. The present study aimed to select individuals with enhanced host-seeking abilities from the Heterorhabditis bacteriophora HBH hybrid strain using an apparatus and the selected individuals were then compared with the original strain in terms of infectivity, reproductive capacity, and storage survival. Initially, last-stage Galleria mellonella larvae were placed in a plastic cage at the bottom of the apparatus. Subsequently, 1000 infective juveniles (IJs) were transferred to the upper part of the apparatus, and kept at 25 °C. This process was repeated until the fifth generation was obtained. The fifth-generation individuals (termed HBH Plus) were then compared with those of the HBH hybrid strain. The results indicated that the highest efficacy of the selected individuals (HBH Plus) against G. mellonella and Tenebrio molitor larvae, Rhizopertha dominica, and Oryzaephilus surinamensis adults was 90, 73.33, 86.67, and 83.33 %, respectively. For the parental culture, these values were 76.67, 63.33, 73.33 and 73.33 %, respectively. When reproductive capacity was examined, HBH Plus producing an average of 125.333,33 IJs per G. mellonella larva, versus 108.741,67 in the parental strain per G. mellonella larva. An increase in mortality rates in the IJ stages was observed with the extension of the storage duration in both the HBH hybrid strain and HBH Plus individuals. HBH Plus reached a mortality rate of 20.21 % by month five. These findings suggest that selection for enhanced host-seeking behavior in EPNs can improve their biological control potential.

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嗜菌异habditis phhora HBH杂交菌株高寻优个体的生物学特性及其生物防治应用

昆虫病原线虫（EPNs）被广泛应用于害虫的生物防治。本研究旨在利用仪器从嗜菌异habditis bacteriophora HBH杂交菌株中筛选具有增强寄主寻找能力的个体，并将所选个体与原始菌株在感染性、繁殖能力和储存存活率方面进行比较。最初，最后阶段的mellonella幼虫被放置在装置底部的一个塑料笼子里。随后，将1000只感染幼虫（IJs）转移到仪器的上部，并在25°C下保存。这个过程一直重复到第五代。然后将第五代个体（称为HBH Plus）与HBH杂交株进行比较。结果表明，HBH Plus对黄粉蚧幼虫、黄粉蚧幼虫、稻瘟虫成虫的最高防效分别为90%、73.33%、86.67%和83.33%。对于亲本培养，这些值分别为76.67%、63.33%、73.33%和73.33%。测定繁殖能力时，HBH Plus菌株平均每只大黄蜂幼虫产生125.3333 ij，亲本菌株平均每只大黄蜂幼虫产生108.741.67 ij。随着贮藏时间的延长，HBH杂交株和HBH Plus个体的IJ期死亡率均有所增加。到第五个月，HBH +的死亡率达到20.21%。这些发现表明，epn选择增强的寻宿主行为可以提高其生物控制潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.