Dual-Responsive fluorinated glycopolymers Targeting GLUT1 for enhanced BBB penetration and Activatable 19F MRI imaging

IF 5.8 2区化学 Q1 POLYMER SCIENCE

European Polymer Journal Pub Date : 2025-06-13 DOI:10.1016/j.eurpolymj.2025.114072

Xuan Liu , Feng Liu , Lu Yuan , Huijun Zhang , Bo Zhao , Xin Wen , Wenjuan Yin , Jie Zhang , Yuangong Zhang , Xinwu Ba , Libin Bai

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引用次数: 0

Abstract

Fluorine-19 magnetic resonance imaging (¹⁹F MRI) holds significant potential for the accurate diagnosis of deep-seated pathological tissues, particularly in brain disorders. However, the application of polymer-based ¹⁹F MRI for brain disorder diagnosis is still limited, mainly due to the restrictive nature of the blood–brain barrier (BBB). In this study, we developed pH- and glutathione (GSH)-responsive hyperbranched fluorinated polymers (HBP-MBEHs) capable of crossing the BBB. The ¹⁹F MRI of HBP-MBEHs had no signal before activation (OFF) and progressively intensified following exposure to acid conditions and GSH stimulation (ON). The glucose moieties in HBP-MBEHs enable crossing the BBB via glucose transporter-1 (GLUT1)-mediated transport, as demonstrated by in vivo small animal fluorescence imaging. Evaluations of cytotoxicity, hemolysis, and hematoxylin and eosin (H&E) staining confirmed the excellent biocompatibility of HBP-MBEHs. This study establishes a noninvasive detection platform for brain disorders.

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靶向GLUT1的双响应氟化糖共聚物增强血脑屏障穿透和可激活的19F MRI成像

氟-19磁共振成像（19F MRI）在准确诊断深层病理组织，特别是脑部疾病方面具有重要潜力。然而，基于聚合物的19F MRI在脑部疾病诊断中的应用仍然有限，主要是由于血脑屏障（BBB）的限制性。在这项研究中，我们开发了能够穿过血脑屏障的pH和谷胱甘肽（GSH）响应的超支化氟化聚合物（HBP-MBEHs）。HBP-MBEHs的19F MRI在激活前无信号（OFF），在暴露于酸性条件和GSH刺激后逐渐增强（ON）。体内小动物荧光成像显示，HBP-MBEHs中的葡萄糖部分能够通过葡萄糖转运蛋白-1 （GLUT1）介导的运输穿越血脑屏障。细胞毒性、溶血、苏木精和伊红（H&；E）染色证实HBP-MBEHs具有良好的生物相容性。本研究建立了一个无创的脑部疾病检测平台。

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来源期刊

European Polymer Journal 化学-高分子科学

CiteScore

9.90

自引率

10.00%

发文量

691

审稿时长

23 days

期刊介绍： European Polymer Journal is dedicated to publishing work on fundamental and applied polymer chemistry and macromolecular materials. The journal covers all aspects of polymer synthesis, including polymerization mechanisms and chemical functional transformations, with a focus on novel polymers and the relationships between molecular structure and polymer properties. In addition, we welcome submissions on bio-based or renewable polymers, stimuli-responsive systems and polymer bio-hybrids. European Polymer Journal also publishes research on the biomedical application of polymers, including drug delivery and regenerative medicine. The main scope is covered but not limited to the following core research areas: Polymer synthesis and functionalization • Novel synthetic routes for polymerization, functional modification, controlled/living polymerization and precision polymers. Stimuli-responsive polymers • Including shape memory and self-healing polymers. Supramolecular polymers and self-assembly • Molecular recognition and higher order polymer structures. Renewable and sustainable polymers • Bio-based, biodegradable and anti-microbial polymers and polymeric bio-nanocomposites. Polymers at interfaces and surfaces • Chemistry and engineering of surfaces with biological relevance, including patterning, antifouling polymers and polymers for membrane applications. Biomedical applications and nanomedicine • Polymers for regenerative medicine, drug delivery molecular release and gene therapy The scope of European Polymer Journal no longer includes Polymer Physics.