An ultrasensitive electrochemical biosensor for ATP detection in human serum based on tetrahedral nanostructured peptides and exonuclease I-assisted signal amplification strategy
Luyu Sun, Yaxuan Zuo, Yunpeng Lu, Jiasheng Wang, Ni Hui
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引用次数: 0
Abstract
Adenosine triphosphate (ATP) is intricately associated to various pathophysiological processes, serving as a crucial energy source for cellular activities. However, the development of a sensitive ATP detection method applicable in complex media presents significant challenges owing to severe biofouling. Herein, a robust antifouling biosensor was developed by utilizing tetrahedral nanostructured peptides containing D-amino acids (D-TPEP) in conjunction with DNA strands for the detection of ATP. The synthesized MXene-doped poly(3,4-ethylenedioxythiophene) (PEDOT) nanocomposites effectively enhanced the conductivity of the electrode and provided an efficient platform for the immobilization of D-TPEP/DNA conjugate. Specifically, D-TPEP was engineered to connect complementary DNA (cDNA), while probe DNA (pDNA) was incorporated through the complementary base pairing, thereby facilitating the formation of double-stranded DNA (dsDNA). By targeting the ATP model, pDNA was induced to detach from the dsDNA through specific binding and subsequently entered the exonuclease I-mediated target recycling process, resulting in signal amplification. Furthermore, the integration of D-TPEP/DNA conjugate with PEDOT/MXene nanocomposite enhanced the anti-biofouling ability of the biosensor, thereby facilitating direct detection of serum samples without any pretreatment. The biosensor demonstrated a relatively broad linear range from 0.1 fM to 1 nM, with a detection limit (LOD) as low as 0.07 fM. Consequently, this study successfully accomplished the ultrasensitive detection of ATP in biological environments and established a strategy for the development of low-fouling biosensors applicable to clinical diagnostics.
期刊介绍:
Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.