{"title":"Homeobox C6 plays an oncogenic role in bladder cancer.","authors":"Ding-Jin Lu, Hai-Rong Wang, You-Sheng Xu, Hai-Bo Huang, Qi-Gang Zhong, Yuan-Ning Luo, Jian-Feng Qi, Hong-Chao Wu, Jin-Ye Pei, Kun Zhang, Chao-Xiong Xu, Tian-Xian Wang, Wei Zhang, Yu-Hong Zhou, Zhi-Guang Huang, Fu-Bo Wang","doi":"10.5306/wjco.v16.i5.103830","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Bladder cancer (BLCA) is a common urological tumor. Homeobox C6 (<i>HOXC6</i>) is an HOX family gene that has an oncogenic effect in various malignancies.</p><p><strong>Aim: </strong>To investigate the expression and function of <i>HOXC6</i> in BLCA.</p><p><strong>Methods: </strong>This study employed immunohistochemistry, along with global chip and sequencing data for BLCA, to comprehensively evaluate the protein and mRNA expression of <i>HOXC6</i> in BLCA. RNA interference technology was employed to knock down the mRNA expression of <i>HOXC6</i> in BLCA cells, and the impact of reduced <i>HOXC6</i> expression on cellular function was assessed. Additionally, we explored the potential mechanisms of <i>HOXC6</i> in BLCA by aggregating <i>HOXC6</i> chromatin immunoprecipitation sequencing data.</p><p><strong>Results: </strong>The immunohistochemistry results, sequencing data, and microarray data revealed that both the mRNA and protein expressions of <i>HOXC6</i> in BLCA were notably greater than the expressions in non-cancerous tissues. Knocking down the expression of <i>HOXC6</i> considerably limited the function of cell proliferation, migration, and invasion abilities of BLCA cells, elevated cell apoptosis, and triggered the G0/G1 phase blockade. The potential target genes of <i>HOXC6</i> were enriched in pathways such as chemical carcinogenesis and reactive oxygen species. A notable positive correlation between <i>HOXC6</i> mRNA expression and its target gene timeless circadian regulator (<i>TIMELESS</i>) was revealed. Notable binding peak signals for <i>HOXC6</i> were identified in the promoter region of <i>TIMELESS</i>.</p><p><strong>Conclusion: </strong><i>HOXC6</i> is upregulated in BLCA and may influence the cellular functions of BLCA by regulating the expression of the target gene <i>TIMELESS</i>.</p>","PeriodicalId":23802,"journal":{"name":"World journal of clinical oncology","volume":"16 5","pages":"103830"},"PeriodicalIF":2.6000,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149837/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"World journal of clinical oncology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5306/wjco.v16.i5.103830","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Bladder cancer (BLCA) is a common urological tumor. Homeobox C6 (HOXC6) is an HOX family gene that has an oncogenic effect in various malignancies.
Aim: To investigate the expression and function of HOXC6 in BLCA.
Methods: This study employed immunohistochemistry, along with global chip and sequencing data for BLCA, to comprehensively evaluate the protein and mRNA expression of HOXC6 in BLCA. RNA interference technology was employed to knock down the mRNA expression of HOXC6 in BLCA cells, and the impact of reduced HOXC6 expression on cellular function was assessed. Additionally, we explored the potential mechanisms of HOXC6 in BLCA by aggregating HOXC6 chromatin immunoprecipitation sequencing data.
Results: The immunohistochemistry results, sequencing data, and microarray data revealed that both the mRNA and protein expressions of HOXC6 in BLCA were notably greater than the expressions in non-cancerous tissues. Knocking down the expression of HOXC6 considerably limited the function of cell proliferation, migration, and invasion abilities of BLCA cells, elevated cell apoptosis, and triggered the G0/G1 phase blockade. The potential target genes of HOXC6 were enriched in pathways such as chemical carcinogenesis and reactive oxygen species. A notable positive correlation between HOXC6 mRNA expression and its target gene timeless circadian regulator (TIMELESS) was revealed. Notable binding peak signals for HOXC6 were identified in the promoter region of TIMELESS.
Conclusion: HOXC6 is upregulated in BLCA and may influence the cellular functions of BLCA by regulating the expression of the target gene TIMELESS.
期刊介绍：
The WJCO is a high-quality, peer reviewed, open-access journal. The primary task of WJCO is to rapidly publish high-quality original articles, reviews, editorials, and case reports in the field of oncology. In order to promote productive academic communication, the peer review process for the WJCO is transparent; to this end, all published manuscripts are accompanied by the anonymized reviewers’ comments as well as the authors’ responses. The primary aims of the WJCO are to improve diagnostic, therapeutic and preventive modalities and the skills of clinicians and to guide clinical practice in oncology. Scope: Art of Oncology, Biology of Neoplasia, Breast Cancer, Cancer Prevention and Control, Cancer-Related Complications, Diagnosis in Oncology, Gastrointestinal Cancer, Genetic Testing For Cancer, Gynecologic Cancer, Head and Neck Cancer, Hematologic Malignancy, Lung Cancer, Melanoma, Molecular Oncology, Neurooncology, Palliative and Supportive Care, Pediatric Oncology, Surgical Oncology, Translational Oncology, and Urologic Oncology.
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