The oral microbiome of root caries: A scoping review

IF 4.8 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of dentistry Pub Date : 2025-06-10 DOI:10.1016/j.jdent.2025.105899

Ronnie Ruonan Zhang, Josie Shizhen Zhang, Shi Huang, Walter Yu-Hang Lam, Chun-Hung Chu, Ollie Yiru Yu

{"title":"The oral microbiome of root caries: A scoping review","authors":"Ronnie Ruonan Zhang, Josie Shizhen Zhang, Shi Huang, Walter Yu-Hang Lam, Chun-Hung Chu, Ollie Yiru Yu","doi":"10.1016/j.jdent.2025.105899","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>This review characterizes shifts of the oral microbial community on carious root surfaces compared to sound root surfaces.</div></div><div><h3>Data and sources</h3><div>A systematic search of English-language publications on clinical studies evaluating oral microbiomes in patients with root caries using high-throughput sequencing technologies published before April 1, 2025, was included. The search was conducted on PubMed, Cochrane Central Register of Controlled Trials, Web of Science, Embase, and Scopus. Gray literature was searched in ClinicalTrials.gov and Google Scholar.</div></div><div><h3>Study selection/Results</h3><div>Based on the eligibility criteria, 1133 publications were screened, and 465 duplicates were removed. Of the remaining 16 studies assessed for full-text review, eight investigating the oral microbiome of saliva, carious roots, or dental plaque in patients with root caries were included. These studies reported the intra-community species diversity (alpha-diversity, 4/8 studies), inter-community compositional diversity (beta-diversity, 4/8 studies), dominant microbial genera/species (8/8 studies), and functional pathways (1/8 studies) of the microbial community in root caries patients. Alpha-diversity showed no significant difference between root caries and sound root surfaces in three studies, but root caries exhibited a significantly lower alpha diversity in one study. Beta-diversity differed significantly between root caries and sound root surfaces in three studies, with one study reporting no difference. The dominant microbial species in root caries varied among the included studies. However, <em>Lactobacillus</em> spp., <em>Prevotella denticola, Propionibacterium acidifaciens, Streptococcus mutans</em>, and <em>Veillonella parvula/dispar</em> were frequently identified in the root caries-associated microbiota. Furthermore, root caries-associated bacteria altered the predicted functional pathways, promoting organic acid production and accelerating collagen degradation.</div></div><div><h3>Conclusion</h3><div>Root caries microbiomes exhibit distinct compositional profiles, dysbiotic species predominance, and a shift in predicted functional pathways compared to healthy root surfaces.</div></div><div><h3>Clinical significance</h3><div>This review provides valuable insights into root caries’ microbial landscape, potentially guiding future preventative and therapeutic strategies.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"160 ","pages":"Article 105899"},"PeriodicalIF":4.8000,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of dentistry","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0300571225003434","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

This review characterizes shifts of the oral microbial community on carious root surfaces compared to sound root surfaces.

Data and sources

A systematic search of English-language publications on clinical studies evaluating oral microbiomes in patients with root caries using high-throughput sequencing technologies published before April 1, 2025, was included. The search was conducted on PubMed, Cochrane Central Register of Controlled Trials, Web of Science, Embase, and Scopus. Gray literature was searched in ClinicalTrials.gov and Google Scholar.

Study selection/Results

Based on the eligibility criteria, 1133 publications were screened, and 465 duplicates were removed. Of the remaining 16 studies assessed for full-text review, eight investigating the oral microbiome of saliva, carious roots, or dental plaque in patients with root caries were included. These studies reported the intra-community species diversity (alpha-diversity, 4/8 studies), inter-community compositional diversity (beta-diversity, 4/8 studies), dominant microbial genera/species (8/8 studies), and functional pathways (1/8 studies) of the microbial community in root caries patients. Alpha-diversity showed no significant difference between root caries and sound root surfaces in three studies, but root caries exhibited a significantly lower alpha diversity in one study. Beta-diversity differed significantly between root caries and sound root surfaces in three studies, with one study reporting no difference. The dominant microbial species in root caries varied among the included studies. However, Lactobacillus spp., Prevotella denticola, Propionibacterium acidifaciens, Streptococcus mutans, and Veillonella parvula/dispar were frequently identified in the root caries-associated microbiota. Furthermore, root caries-associated bacteria altered the predicted functional pathways, promoting organic acid production and accelerating collagen degradation.

Conclusion

Root caries microbiomes exhibit distinct compositional profiles, dysbiotic species predominance, and a shift in predicted functional pathways compared to healthy root surfaces.

Clinical significance

This review provides valuable insights into root caries’ microbial landscape, potentially guiding future preventative and therapeutic strategies.

查看原文本刊更多论文

牙根龋的口腔微生物组：综述。

目的：本文综述了口腔微生物群落在龋齿根表面与正常根表面的变化。数据和来源：系统检索了2025年4月1日之前发表的关于使用高通量测序技术评估牙根龋患者口腔微生物组临床研究的英文出版物。检索是在PubMed， Cochrane Central Register of Controlled Trials, Web of Science， Embase和Scopus上进行的。灰色文献在ClinicalTrials.gov和谷歌Scholar上检索。研究选择/结果：根据入选标准，筛选了1133篇文献，删除了465篇重复文献。在剩余的16项研究中，有8项研究调查了牙根龋患者的唾液、龋根或牙菌斑的口腔微生物组。这些研究报道了根龋患者微生物群落的群落内物种多样性（α -多样性，4/8项研究）、群落间组成多样性（β -多样性，4/8项研究）、优势微生物属/种（8/8项研究）和功能途径（1/8项研究）。3项研究中，根龋与健全根面α多样性差异不显著，但有1项研究中，根龋α多样性显著降低。在三项研究中，β -多样性在根龋和健全根表面之间存在显著差异，其中一项研究报告没有差异。在纳入的研究中，根龋的优势微生物种类有所不同。然而，乳酸菌、牙齿普雷沃氏菌、增酸丙酸杆菌、变形链球菌和细小微孔菌在与牙根龋相关的微生物群中经常被发现。此外，与牙根龋相关的细菌改变了预测的功能途径，促进有机酸的产生和加速胶原蛋白的降解。结论：与健康的根表面相比，根龋微生物组表现出不同的组成特征，生态失调的物种优势，以及预测功能途径的转变。临床意义：本综述对牙根龋的微生物景观研究提供了有价值的见解，可能指导未来的预防和治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.