Detection of HIV-1 Antibodies in Saliva of Persons Living With HIV Using Blood-Based First Response HIV 1-2.O Card Test.

IF 2.9 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2025-08-01 Epub Date: 2025-06-12 DOI:10.1002/jcla.70069

Enoch Aninagyei, Comfort Addo Boatey, Gifty Larbi, Wilson Bright Tsidi, Raphael Eyram Amemo, Ebenezer Tawiah Nyarkotey, Godknows Afenya, Desmond Omane Acheampong

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Abstract

Background: This study tested HIV-1 antibodies in saliva samples (sHIV-1 Ab) collected by the Self-Lollisponge device.

Methods: Blood and saliva from confirmed persons with HIV and HIV-negative controls were analyzed for HIV-1/2 antibodies using the blood-based First Response HIV 1-2.O Card Test. The sampling device containing sHIV-1 Ab was stored at 6°C for 60 days, with intermittent testing on days 2, 5, 10, 20, 30, and 60. Regression analysis was done to assess the relationship between the presence of sHIV-1 Ab and independent variables.

Results: The sensitivity and the specificity of detecting sHIV-1 Ab were 72.9% (95% CI: 63.92%-80.65%) and 100% (95% CI: 92.89%-100.00%), respectively. The presence of opportunistic infections (AOR = 13.1, p < 0.001), having stomatorrhagia (AOR = 4.56, p = 0.0022), and hyperviremia (> 201 copies/mL) (AOR = 4.91, p = 0.0225) heightened sHIV-1 Ab detection. Furthermore, fatigue (AOR = 12.1, p = 0.0024), fever (AOR = 3.5, p = 0.0144), and weight loss (AOR = 10.9, p = 0.0318) increased the odds of having sHIV-1 Ab in persons living with HIV (PLWHIV). sHIV-1 Ab was identified in over 90% of PLWHIV with opportunistic infections (OIs) and stomatorrhagia, OIs and hyperviremia, and stomatorrhagia and hyperviremia. Upon storage for 60 days, the sHIV-1 Ab was detected in all the samples.

Conclusion: Saliva could be an alternative to blood for diagnosing HIV. In addition, the Self-Lollisponge device was found to be user-friendly, acquiescent to all settings, and cheap, and can preserve sHIV-1 Ab for at least 60 days.

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血液第一反应HIV- 1-2检测HIV-1抗体O卡片测试。

背景：本研究检测了通过self - lolisponge装置收集的唾液样本中的HIV-1抗体（sHIV-1 Ab）。方法：采用基于血液的第一反应HIV- 1-2对确诊HIV感染者和HIV阴性对照组的血液和唾液进行HIV-1/2抗体检测。O卡片测试。含有sHIV-1 Ab的取样装置在6℃下保存60天，在第2、5、10、20、30、60天进行间歇检测。回归分析评估shiv - 1ab的存在与自变量的关系。结果：检测sHIV-1 Ab的灵敏度为72.9% (95% CI: 63.92% ~ 80.65%)，特异性为100% （95% CI: 92.89% ~ 100.00%）。机会性感染（AOR = 13.1， p 201拷贝/mL）（AOR = 4.91, p = 0.0225）使sHIV-1抗体检测升高。此外，疲劳（AOR = 12.1, p = 0.0024）、发热（AOR = 3.5, p = 0.0144）和体重减轻（AOR = 10.9, p = 0.0318）增加了HIV感染者感染sHIV-1 Ab的几率。sHIV-1抗体在90%以上的plwhv机会性感染（OIs）伴口出血、OIs伴高病毒血症和口出血伴高病毒血症中被鉴定出来。保存60天后，所有样品均检测到sHIV-1抗体。结论：唾液可作为HIV诊断的替代血液。此外，self - lolisponge设备被发现是用户友好的，默认所有设置，价格便宜，并且可以保存sHIV-1 Ab至少60天。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.