The Prognostic Significance of TMSB4X in Glioma Patients.

IF 2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

International Journal of General Medicine Pub Date : 2025-06-06 eCollection Date: 2025-01-01 DOI:10.2147/IJGM.S521390

Sijie Li, Tianyi Fan, Zengyao Hao, Zizhou Zhang, Xuetao Han, Jiayuan Li, Yu Wang, Huandi Zhou, Xiaoying Xue

{"title":"The Prognostic Significance of TMSB4X in Glioma Patients.","authors":"Sijie Li, Tianyi Fan, Zengyao Hao, Zizhou Zhang, Xuetao Han, Jiayuan Li, Yu Wang, Huandi Zhou, Xiaoying Xue","doi":"10.2147/IJGM.S521390","DOIUrl":null,"url":null,"abstract":"Objective: The goal of this study was to analyze in depth the importance of the thymosin beta 4 X-linked gene (TMSB4X) in the disease process of gliomas for the prediction of patient prognosis.Methods: We explored the expression of TMSB4X by analyzing datasets from The Cancer Genome Atlas (TCGA) datasets and the Chinese Glioma Genome Atlas (CGGA). Tumor sample tissues and corresponding paracancerous tissues of glioma patients were collected, and TMSB4X expression in glioma tissues was analyzed using Real-Time PCR to investigate its prognostic significance in glioma patients. In addition, we have plotted the ROC curves for the survival rate, performed univariate and multivariate Cox analyses and constructed nomogram. Differential expression analyses were performed on the basis of median expression levels of the TMSB4X gene, and Intuitive graphical presentation and visualisation of the 403 proteins screened were carried out using the Cytoscape software platform. (version: 3.9.1). The top 50 core genes were then screened using the cyto Hubba algorithm, these genes were analyzed using The Gene Ontology Database (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). TCGA datasets was utilized for Gene Set Enrichment Analysis (GSEA) to obtain potential NF-κB-related pathways in gliomas.Results: Independent prognostic analyses show that the TMSB4X gene was identified as an independent prognostic indicator for gliomas. In GOKEGG and GSEA analyses, the analyses indicate that TMSB4X may play a crucial role in glioma patients by up-regulating I-kappaB kinase/NF-kappaB signaling. In glioma patients, upregulation of the I-kappaB kinase and NF-kappaB signalling pathways plays a crucial role, which may be linked to CASP1.Conclusion: The findings showed that TMSB4X was identified as an potential independent risk factor in the prognosis of glioma patients, a finding that implies that TMSB4X has the potential to serve as a key biomarker for predicting the prognostic status of glioma patients.","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"18 ","pages":"2923-2940"},"PeriodicalIF":2.0000,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152311/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of General Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/IJGM.S521390","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: The goal of this study was to analyze in depth the importance of the thymosin beta 4 X-linked gene (TMSB4X) in the disease process of gliomas for the prediction of patient prognosis.

Methods: We explored the expression of TMSB4X by analyzing datasets from The Cancer Genome Atlas (TCGA) datasets and the Chinese Glioma Genome Atlas (CGGA). Tumor sample tissues and corresponding paracancerous tissues of glioma patients were collected, and TMSB4X expression in glioma tissues was analyzed using Real-Time PCR to investigate its prognostic significance in glioma patients. In addition, we have plotted the ROC curves for the survival rate, performed univariate and multivariate Cox analyses and constructed nomogram. Differential expression analyses were performed on the basis of median expression levels of the TMSB4X gene, and Intuitive graphical presentation and visualisation of the 403 proteins screened were carried out using the Cytoscape software platform. (version: 3.9.1). The top 50 core genes were then screened using the cyto Hubba algorithm, these genes were analyzed using The Gene Ontology Database (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). TCGA datasets was utilized for Gene Set Enrichment Analysis (GSEA) to obtain potential NF-κB-related pathways in gliomas.

Results: Independent prognostic analyses show that the TMSB4X gene was identified as an independent prognostic indicator for gliomas. In GOKEGG and GSEA analyses, the analyses indicate that TMSB4X may play a crucial role in glioma patients by up-regulating I-kappaB kinase/NF-kappaB signaling. In glioma patients, upregulation of the I-kappaB kinase and NF-kappaB signalling pathways plays a crucial role, which may be linked to CASP1.

Conclusion: The findings showed that TMSB4X was identified as an potential independent risk factor in the prognosis of glioma patients, a finding that implies that TMSB4X has the potential to serve as a key biomarker for predicting the prognostic status of glioma patients.

查看原文本刊更多论文

脑胶质瘤患者TMSB4X的预后意义。

目的：深入分析胸腺素- 4x -连锁基因（TMSB4X）在胶质瘤发病过程中的重要性，预测患者预后。方法：通过分析肿瘤基因组图谱（TCGA）和中国胶质瘤基因组图谱（CGGA）的数据，探讨TMSB4X的表达。收集胶质瘤患者的肿瘤样本组织及相应的癌旁组织，采用Real-Time PCR分析胶质瘤组织中TMSB4X的表达，探讨其在胶质瘤患者中的预后意义。此外，我们绘制了生存率的ROC曲线，进行了单因素和多因素Cox分析，并构建了nomogram。根据TMSB4X基因的中位表达水平进行差异表达分析，并使用Cytoscape软件平台对筛选的403个蛋白进行直观的图形呈现和可视化。(版本:3.9.1)。然后使用cyto Hubba算法筛选前50个核心基因，使用基因本体数据库（GO）和京都基因与基因组百科全书（KEGG）对这些基因进行分析。利用TCGA数据集进行基因集富集分析（GSEA），以获得胶质瘤中潜在的NF-κ b相关通路。结果：独立预后分析表明，TMSB4X基因被确定为胶质瘤的独立预后指标。在GOKEGG和GSEA分析中，分析表明TMSB4X可能通过上调I-kappaB激酶/NF-kappaB信号通路在胶质瘤患者中发挥关键作用。在胶质瘤患者中，I-kappaB激酶和NF-kappaB信号通路的上调起着至关重要的作用，这可能与CASP1有关。结论：TMSB4X被确定为胶质瘤患者预后的潜在独立危险因素，提示TMSB4X有潜力作为预测胶质瘤患者预后状况的关键生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of General Medicine Medicine-General Medicine

自引率

0.00%

发文量

1113

审稿时长

16 weeks

期刊介绍： The International Journal of General Medicine is an international, peer-reviewed, open access journal that focuses on general and internal medicine, pathogenesis, epidemiology, diagnosis, monitoring and treatment protocols. The journal is characterized by the rapid reporting of reviews, original research and clinical studies across all disease areas. A key focus of the journal is the elucidation of disease processes and management protocols resulting in improved outcomes for the patient. Patient perspectives such as satisfaction, quality of life, health literacy and communication and their role in developing new healthcare programs and optimizing clinical outcomes are major areas of interest for the journal. As of 1st April 2019, the International Journal of General Medicine will no longer consider meta-analyses for publication.