Individual muscle hypertrophy response is affected by the overload progression model and is associated with changes in satellite cell content.

IF 2.7 3区医学 Q2 PHYSIOLOGY

European Journal of Applied Physiology Pub Date : 2025-06-11 DOI:10.1007/s00421-025-05817-y

Maíra C Scarpelli, João G A Bergamasco, Joshua S Godwin, Paulo H C Mesquita, Talisson S Chaves, Deivid G Silva, Diego Bittencourt, Nathalia F Dias, Ricardo A Medalha Junior, Paulo C Carello Filho, Vitor Angleri, Luiz A R Costa, Andreas N Kavazis, Carlos Ugrinowitsch, Michael D Roberts, Cleiton A Libardi

{"title":"Individual muscle hypertrophy response is affected by the overload progression model and is associated with changes in satellite cell content.","authors":"Maíra C Scarpelli, João G A Bergamasco, Joshua S Godwin, Paulo H C Mesquita, Talisson S Chaves, Deivid G Silva, Diego Bittencourt, Nathalia F Dias, Ricardo A Medalha Junior, Paulo C Carello Filho, Vitor Angleri, Luiz A R Costa, Andreas N Kavazis, Carlos Ugrinowitsch, Michael D Roberts, Cleiton A Libardi","doi":"10.1007/s00421-025-05817-y","DOIUrl":null,"url":null,"abstract":"Purpose: We aimed to compare individual hypertrophic responses to resistance training in which overload progressed either by adjusting the load (LOADProg) or by increasing the number of repetitions (REPSProg). Furthermore, we investigated whether greater responsiveness to one protocol was associated with chronic changes in myonuclei and satellite cells, proteolysis and extracellular matrix (ECM) remodeling biomarkers.Methods: Thirty-seven untrained participants had their legs randomized into LOADProg and REPSProg and underwent 10 weeks of training. Muscle cross-sectional area (mCSA) ultrasound and muscle biopsies were performed pre- and post-training. Based on mCSA changes between protocols, we applied a criterion of 2 typical errors (5.7%) to create 4 clusters.Results: Twelve participants (~ 34%) showed greater mCSA increases after REPSProg (14.2 ± 7.6%) than LOADProg (3.4 ± 8.7%, p = 0.004). Seven participants (~ 19%) responded better to LOADProg (21.5 ± 7.5% vs. 12 ± 7.5%, p = 0.041). Thirteen participants (~ 35%) showed no differences between protocols (p = 0.852). Five participants were nonresponders (mCSA changes smaller than the 5.7% threshold) for both protocols. There were no significant differences (p > 0.05) in myonuclear content, proteolysis, or ECM remodeling markers within any of the clusters. However, for those who responded better to REPSProg, this protocol promoted greater satellite cell changes (108.6 ± 77.0%) than LOADProg (48.9 ± 63.1%, p = 0.015).Conclusion: Our findings suggest that overload progression models may influence individual responsiveness to RT-induced muscle hypertrophy. Additionally, progression through increased repetitions was associated with a chronic addition of satellite cells. However, responsiveness was not explained by chronic changes in myonuclei, proteolysis or ECM remodeling biomarkers.Trial registration: This study is registered in the Brazilian Registry of Clinical Trials (RBR-57v9mrb).","PeriodicalId":12005,"journal":{"name":"European Journal of Applied Physiology","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Applied Physiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00421-025-05817-y","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHYSIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: We aimed to compare individual hypertrophic responses to resistance training in which overload progressed either by adjusting the load (LOADProg) or by increasing the number of repetitions (REPSProg). Furthermore, we investigated whether greater responsiveness to one protocol was associated with chronic changes in myonuclei and satellite cells, proteolysis and extracellular matrix (ECM) remodeling biomarkers.

Methods: Thirty-seven untrained participants had their legs randomized into LOADProg and REPSProg and underwent 10 weeks of training. Muscle cross-sectional area (mCSA) ultrasound and muscle biopsies were performed pre- and post-training. Based on mCSA changes between protocols, we applied a criterion of 2 typical errors (5.7%) to create 4 clusters.

Results: Twelve participants (~ 34%) showed greater mCSA increases after REPSProg (14.2 ± 7.6%) than LOADProg (3.4 ± 8.7%, p = 0.004). Seven participants (~ 19%) responded better to LOADProg (21.5 ± 7.5% vs. 12 ± 7.5%, p = 0.041). Thirteen participants (~ 35%) showed no differences between protocols (p = 0.852). Five participants were nonresponders (mCSA changes smaller than the 5.7% threshold) for both protocols. There were no significant differences (p > 0.05) in myonuclear content, proteolysis, or ECM remodeling markers within any of the clusters. However, for those who responded better to REPSProg, this protocol promoted greater satellite cell changes (108.6 ± 77.0%) than LOADProg (48.9 ± 63.1%, p = 0.015).

Conclusion: Our findings suggest that overload progression models may influence individual responsiveness to RT-induced muscle hypertrophy. Additionally, progression through increased repetitions was associated with a chronic addition of satellite cells. However, responsiveness was not explained by chronic changes in myonuclei, proteolysis or ECM remodeling biomarkers.

Trial registration: This study is registered in the Brazilian Registry of Clinical Trials (RBR-57v9mrb).

查看原文本刊更多论文

个体肌肉肥大反应受过载进展模型的影响，并与卫星细胞含量的变化有关。

目的：我们的目的是比较个体对阻力训练的肥壮反应，其中负荷通过调整负荷（LOADProg）或增加重复次数（REPSProg）进行。此外，我们研究了对一种方案的更高反应性是否与肌核和卫星细胞、蛋白质水解和细胞外基质（ECM）重塑生物标志物的慢性变化有关。方法：37名未经训练的参与者将他们的腿随机分为LOADProg和REPSProg，并进行10周的训练。训练前后分别进行肌肉横截面积（mCSA）超声检查和肌肉活检。基于协议之间mCSA的变化，我们采用2个典型错误（5.7%）的标准创建了4个集群。结果：12例（~ 34%）受试者在REPSProg后mCSA升高（14.2±7.6%）高于LOADProg（3.4±8.7%,p = 0.004）。7名参与者（~ 19%）对LOADProg的反应更好（21.5±7.5% vs. 12±7.5%,p = 0.041）。13名参与者（~ 35%）在方案之间没有差异（p = 0.852）。5名参与者对两种方案均无反应（mCSA变化小于5.7%阈值）。在任何簇内，心肌核含量、蛋白水解或ECM重塑标志物均无显著差异（p > 0.05）。然而，对于那些对REPSProg反应更好的患者，REPSProg方案比LOADProg方案（48.9±63.1%,p = 0.015）促进了更大的卫星细胞变化（108.6±77.0%）。结论：我们的研究结果表明，超负荷进展模型可能影响个体对rt诱导的肌肉肥大的反应性。此外，通过增加重复的进展与卫星细胞的慢性增加有关。然而，反应性不能用肌核、蛋白水解或ECM重塑生物标志物的慢性变化来解释。试验注册：本研究已在巴西临床试验注册中心（RBR-57v9mrb）注册。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

European Journal of Applied Physiology 医学-生理学

CiteScore

6.00

自引率

6.70%

发文量

227

审稿时长

3 months

期刊介绍： The European Journal of Applied Physiology (EJAP) aims to promote mechanistic advances in human integrative and translational physiology. Physiology is viewed broadly, having overlapping context with related disciplines such as biomechanics, biochemistry, endocrinology, ergonomics, immunology, motor control, and nutrition. EJAP welcomes studies dealing with physical exercise, training and performance. Studies addressing physiological mechanisms are preferred over descriptive studies. Papers dealing with animal models or pathophysiological conditions are not excluded from consideration, but must be clearly relevant to human physiology.