Enzyme-assisted recovery of phenolic compounds from broccoli waste with antioxidant and antihyperglycemic activities evaluated through in vitro and molecular docking studies

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-06-07 DOI:10.1016/j.bcab.2025.103632

Carlos N. Cano-Gonzalez , Rocio Yaneli Aguirre-Loredo , Juan Alberto Ascacio Valdes , Cristobal N. Aguilar González , Lluvia de Abril Alexandra Soriano-Melgar

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引用次数: 0

Abstract

The zero-waste strategy encompasses several Sustainable Development Goals, focusing on using and managing resources to produce value-added compounds. Broccoli waste represents a valuable and underutilized source of phenolic compounds, renowned for their diverse bioactive properties. This study employed an innovative enzyme-assisted extraction (EAE) method as a green and efficient alternative for obtaining these bioactive compounds without compromising their composition and functionality. The objective of this research was to harness sustainable technology to extract phenolic compounds from broccoli waste, maximizing their potential bioactivities. The EAE, specifically the commercial enzymatic preparation (Pectinase with polygalacturonase activity of 100 U g⁻¹), exhibited a high extraction efficiency of phenolic compounds (14.53 mg GAE g⁻¹) and flavonoids (7.63 mg QE g⁻¹), with an antioxidant activity of 95.33 %. Flavonoid glycosides demonstrated molecular docking interactions (≤−8.0 kcal mol⁻¹) with the catalytic amino acids of α-amylase and DPP-4. This provides a scheme to indicate the source and extraction method to obtain compounds of importance as therapeutic alternatives in chronic degenerative diseases. The findings highlight the potential application of these phenolic compounds as functional food additives, capable of scavenging free radicals and regulating postprandial blood glucose levels.

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酶辅助回收西兰花废弃物中酚类化合物及其抗氧化和降血糖活性的体外和分子对接研究

零废物战略包括若干可持续发展目标，重点是利用和管理资源以生产增值化合物。西兰花废料是一种有价值但未充分利用的酚类化合物来源，以其多种生物活性特性而闻名。本研究采用了一种创新的酶辅助提取（EAE）方法，作为一种绿色高效的替代方法，在不影响其组成和功能的情况下获得这些生物活性化合物。本研究的目的是利用可持续技术从西兰花废料中提取酚类化合物，最大限度地发挥其潜在的生物活性。EAE，特别是商业酶制剂（果胶酶的聚半乳糖酶活性为100 U g−1），显示出较高的酚类化合物（14.53 mg GAE g−1）和类黄酮（7.63 mg QE g−1）的提取效率，抗氧化活性为95.33%。类黄酮苷与α-淀粉酶和DPP-4的催化氨基酸表现出分子对接作用（≤−8.0 kcal mol−1）。这提供了一个方案，以表明来源和提取方法，以获得重要的化合物，作为慢性退行性疾病的治疗方案。这些发现强调了这些酚类化合物作为功能性食品添加剂的潜在应用，它们能够清除自由基并调节餐后血糖水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.