Synergy between adsorption and bio-degradation of real wastewater polycyclic aromatic hydrocarbons (PAHs) by laccase immobilized on pineapples waste activated carbon: Recyclability, performance, and DFT analysis

IF 6.7 2区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of water process engineering Pub Date : 2025-06-13 DOI:10.1016/j.jwpe.2025.108124

Osamah J. Al-sareji , Ruqayah Ali Grmasha , Mónika Meiczinger , Miklós Jakab , Csilla Stenger-Kovács , Faisal M. Alfaisal , Tinku Biswas , Mohammed A. Al-Seady , Shamshad Alam , Raed A. Al-Juboori

{"title":"Synergy between adsorption and bio-degradation of real wastewater polycyclic aromatic hydrocarbons (PAHs) by laccase immobilized on pineapples waste activated carbon: Recyclability, performance, and DFT analysis","authors":"Osamah J. Al-sareji , Ruqayah Ali Grmasha , Mónika Meiczinger , Miklós Jakab , Csilla Stenger-Kovács , Faisal M. Alfaisal , Tinku Biswas , Mohammed A. Al-Seady , Shamshad Alam , Raed A. Al-Juboori","doi":"10.1016/j.jwpe.2025.108124","DOIUrl":null,"url":null,"abstract":"<div><div>The contamination of micropollutants in wastewater has become a global issue due to their persistent impact on ecosystems and human health. The enzymatic degradation of polycyclic aromatic hydrocarbons (PAHs) offers a promising, sustainable approach, although it is limited by reduced stability and recycling challenges. In this study, we demonstrated the use of pineapple peel waste as a novel support for the immobilization of laccase (Trametes versicolor) via an adsorption technique (laccase@PPAC). The activated carbon synthesized using potassium hydroxide (PPAC), and laccase@PPAC were characterized through various methods. The laccase@PPAC exhibited excellent performance, achieving maximum adsorption capacities of 270.38 mg/g for benzo[<em>a</em>]pyrene (BaP) and 335.27 mg/g for anthracene (Ant). When combined with enzymatic degradation, total removal reached 98.72 % for BaP and 99.87 % for Ant, corresponding to total degradation-enhanced capacities of 301.21 mg/g and 317.41 mg/g, respectively. Additionally, laccase@PPAC maintained high removal efficiency over 20 reuse cycles. The system showed superior thermostability and pH tolerance compared to free-state laccase. Adsorption kinetics followed the pseudo-first-order model, while equilibrium data were best described by the Langmuir isotherm. This work highlights the potential of pineapple waste-derived activated carbon as a sustainable and effective support for enzyme immobilization in the bioremediation of PAHs-contaminated wastewater.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108124"},"PeriodicalIF":6.7000,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water process engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214714425011961","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}

引用次数: 0

Abstract

The contamination of micropollutants in wastewater has become a global issue due to their persistent impact on ecosystems and human health. The enzymatic degradation of polycyclic aromatic hydrocarbons (PAHs) offers a promising, sustainable approach, although it is limited by reduced stability and recycling challenges. In this study, we demonstrated the use of pineapple peel waste as a novel support for the immobilization of laccase (Trametes versicolor) via an adsorption technique (laccase@PPAC). The activated carbon synthesized using potassium hydroxide (PPAC), and laccase@PPAC were characterized through various methods. The laccase@PPAC exhibited excellent performance, achieving maximum adsorption capacities of 270.38 mg/g for benzo[a]pyrene (BaP) and 335.27 mg/g for anthracene (Ant). When combined with enzymatic degradation, total removal reached 98.72 % for BaP and 99.87 % for Ant, corresponding to total degradation-enhanced capacities of 301.21 mg/g and 317.41 mg/g, respectively. Additionally, laccase@PPAC maintained high removal efficiency over 20 reuse cycles. The system showed superior thermostability and pH tolerance compared to free-state laccase. Adsorption kinetics followed the pseudo-first-order model, while equilibrium data were best described by the Langmuir isotherm. This work highlights the potential of pineapple waste-derived activated carbon as a sustainable and effective support for enzyme immobilization in the bioremediation of PAHs-contaminated wastewater.

Abstract Image

查看原文本刊更多论文

菠萝废活性炭固定化漆酶对实际废水中多环芳烃（PAHs）的吸附和生物降解的协同作用：可回收性、性能和DFT分析

由于废水中微污染物对生态系统和人类健康的持续影响，其污染已成为一个全球性问题。多环芳烃（PAHs）的酶降解提供了一个有前途的、可持续的方法，尽管它受到稳定性降低和回收挑战的限制。在这项研究中，我们展示了菠萝皮废料作为一种新的载体，通过吸附技术固定化漆酶（Trametes versicolor）（laccase@PPAC）。用氢氧化钾（PPAC）合成的活性炭和laccase@PPAC通过各种方法进行了表征。laccase@PPAC对苯并[a]芘（BaP）的最大吸附量为270.38 mg/g，对蒽（Ant）的最大吸附量为335.27 mg/g。结合酶促降解，BaP和Ant的总去除率分别达到98.72%和99.87%，总降解能力分别为301.21 mg/g和317.41 mg/g。此外，laccase@PPAC在20多个重复使用循环中保持了较高的去除效率。与游离态漆酶相比，该体系表现出优异的热稳定性和pH耐受性。吸附动力学符合拟一阶模型，而平衡数据最好用Langmuir等温线来描述。这项工作强调了菠萝废物衍生的活性炭在多环芳烃污染废水的生物修复中作为一种可持续和有效的酶固定化支持的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

10.70

自引率

8.60%

发文量

846

审稿时长

24 days

期刊介绍： The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies