Spatiotemporal regulation of target mRNA cleavage by 21-nt phasiRNAs in maize anthers.

IF 9.4 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-06-17 Epub Date: 2025-06-11 DOI:10.1073/pnas.2422647122

Bi Lian, Tianxiao Huang, Wei Huang, Pengfei Jiang, Weiwei Jin, Yijun Qi

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Abstract

21-nt phasiRNAs are abundantly expressed in anthers and play crucial roles in anther development and male reproduction in grasses. In maize, the target genes that 21-nt phasiRNAs regulate remain largely unknown. Here, we show that 21-nt phasiRNAs direct the cleavage of hundreds of target mRNAs in maize. Interestingly, while 21-nt phasiRNAs in anthers (somatic anther wall cells) do not have obvious preference for a particular nucleotide at the 5' end and target genes implicated in stress response and cellular homeostasis, 21-nt phasiRNAs in male germ cells preferentially initiate with 5' C and target genes related to meiotic processes in a ZmAGO5c-dependent manner. Our results indicate that mRNA cleavage is a conserved mechanism used by 21-nt phasiRNAs for gene regulation in grasses. However, the sequences of 21-nt phasiRNAs and their targets show little conservation in maize and rice, suggesting that 21PHAS loci and 21-nt phasiRNA targets have undergone fast divergence in grasses.

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玉米花药中21-nt相rna对靶mRNA裂解的时空调控。

21-nt相rna在禾草植物花药中大量表达，在花药发育和雄性生殖中起着重要作用。在玉米中，21-nt相rna调控的靶基因在很大程度上仍然未知。在这里，我们发现21-nt phasiRNAs指导玉米中数百种靶mrna的切割。有趣的是，虽然花药（体细胞花药壁细胞）中的21-nt phasiRNAs对5‘端特定核苷酸和与应激反应和细胞稳态相关的靶基因没有明显的偏好，但男性生殖细胞中的21-nt phasiRNAs优先启动与5’ C和减数分裂过程相关的靶基因，以依赖zmago5c的方式启动。我们的研究结果表明，mRNA切割是21-nt相rna在草中用于基因调控的保守机制。然而，21-nt phasiRNA及其靶点序列在玉米和水稻中几乎没有保存，这表明21-nt phasiRNA位点和21-nt phasiRNA靶点在禾草中经历了快速分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Proceedings of the National Academy of Sciences of the United States of America 综合性期刊-综合性期刊

CiteScore

19.00

自引率

0.90%

发文量

3575

审稿时长

2.5 months

期刊介绍： The Proceedings of the National Academy of Sciences (PNAS), a peer-reviewed journal of the National Academy of Sciences (NAS), serves as an authoritative source for high-impact, original research across the biological, physical, and social sciences. With a global scope, the journal welcomes submissions from researchers worldwide, making it an inclusive platform for advancing scientific knowledge.