Single-Cell RNA Sequencing Study on Two Synovial Derived Tumors in the Temporomandibular Joint.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-06-11 DOI:10.1111/odi.70003

Weihua Han, Tiansong Xu, Zonghan He, Haiyan Luo, Chuanbin Guo, Juanhong Meng

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引用次数: 0

Abstract

Objective: To investigate the cellular composition and differentiation trajectory of synovial chondromatosis (SC) and diffuse tenosynovial giant cell tumor (D-TSGCT) in the temporomandibular joint (TMJ).

Materials and methods: Single-cell RNA sequencing was applied to analyze the cellular composition, differentiation trajectory, and intercellular communication of SC and D-TSGCT. Cell culture and morphological experiments were applied to validate the chondrogenic differentiation potential of fibroblast-like synoviocytes (FLS) in the initial stage of SC and to explore the expression and regulation of differential genes during chondrogenic differentiation.

Results: SC was mainly composed of FLS and chondrocytes, while D-TSGCT had a relatively complex cellular composition. Genetically, chondrogenesis-related genes such as COMP and SMAD2 were upregulated in the early stage of SC. TGFβ pathway and collagen catabolic process were subsequently activated, ultimately leading to cartilage formation. CSF1 and CD68 were overexpressed at the forming stage of D-TSGCT, and the macrophage pathway was enriched in the later phase. Based on experimental verification, COMP could induce chondrogenic differentiation of FLS by activating TGFβ/SMAD signal pathways in vitro.

Conclusions: FLS are the common starting point of synovial-derived tumors, but differentiate toward different endpoints in the SC and D-TSGCT. COMP/TGFβ/SMAD signaling pathways can promote chondrogenic differentiation of synovium in SC.

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两种颞下颌关节滑膜衍生肿瘤的单细胞RNA测序研究。

目的：探讨颞下颌关节（TMJ）滑膜软骨瘤病（SC）和弥漫性腱鞘巨细胞瘤（D-TSGCT）的细胞组成及分化轨迹。材料和方法：采用单细胞RNA测序技术分析SC和D-TSGCT的细胞组成、分化轨迹和细胞间通讯。通过细胞培养和形态学实验验证SC初始阶段成纤维细胞样滑膜细胞（FLS）的成软骨分化潜能，探讨成软骨分化过程中差异基因的表达和调控。结果：SC主要由FLS和软骨细胞组成，而D-TSGCT的细胞组成相对复杂。在遗传学上，软骨形成相关基因如COMP和SMAD2在SC早期上调，tgf - β通路和胶原分解代谢过程随后被激活，最终导致软骨形成。D-TSGCT形成阶段CSF1和CD68过表达，后期巨噬细胞通路富集。经实验验证，COMP可通过体外激活TGFβ/SMAD信号通路诱导FLS成软骨分化。结论：FLS是滑膜源性肿瘤的共同起点，但在SC和D-TSGCT中分化为不同的终点。COMP/TGFβ/SMAD信号通路可促进SC滑膜软骨分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.