Optimization and improvement of the disc diffusion method for mucoid Pseudomonas aeruginosa.

IF 3.9 2区医学 Q1 INFECTIOUS DISEASES

Journal of Antimicrobial Chemotherapy Pub Date : 2025-06-11 DOI:10.1093/jac/dkaf179

Aixin Wang, Pei Liu, Yun Wu, Ruirui Ma, Wei Kang, Yingchun Xu, Yali Liu

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引用次数: 0

Abstract

Objectives: Antibiotic susceptibility test for mucoid Pseudomonas aeruginosa is challenging to conduct using the disc diffusion method based on Mueller-Hinton agar (MHA) due to its slow growth. The aim of this study is to compare the suitability of blood MHA versus MHA for disc diffusion antimicrobial susceptibility testing of mucoid P. aeruginosa and to determine the optimal reporting time.

Methods: The study employed blood MHA for the disc diffusion method, using MHA as a control, and broth microdilution as a reference method. We tested 60 mucoid P. aeruginosa strains on both media types, with results assessed at 16, 18, 20, 24 and 48 hours.

Results: The findings indicated that the interpretive categories determined by using blood MHA were comparable to those obtained with standard MHA. Notably, compared with the reference method, the categorical agreement for the blood MHA at multiple time points was significantly better than that for the standard MHA. For most tested antibiotics, results could be reliably interpreted at 20-24 hours by using blood MHA, with 24 hours being the optimal interpretation time. Results for quinolones particularly levofloxacin, were unacceptable when compared with reference methods. Additionally, the study found that blood agar and chocolate agar could help reduce the expression of mucus and accelerate its growth rate.

Conclusions: In conclusion, the blood MHA can serve as a substitute for the MHA, with an optimal interpretation time of 24 hours.

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粘液样铜绿假单胞菌圆盘扩散法的优化与改进。

目的：粘液样铜绿假单胞菌生长缓慢，采用基于Mueller-Hinton琼脂（MHA）的圆盘扩散法对其进行药敏试验具有挑战性。本研究的目的是比较血液MHA与MHA在粘液样铜绿假单胞菌（P. aeruginosa）盘片扩散抗菌药敏试验中的适用性，并确定最佳报告时间。方法：采用血MHA作圆盘扩散法，以MHA为对照，肉汤微量稀释法为参比法。我们在两种培养基上测试了60株粘液样铜绿假单胞菌菌株，并在16、18、20、24和48小时评估了结果。结果：研究结果表明，用血液MHA确定的解释类别与用标准MHA获得的解释类别相当。值得注意的是，与参考方法相比，多个时间点血液MHA的分类一致性明显优于标准MHA。对于大多数测试的抗生素，使用血液MHA可以在20-24小时可靠地解释结果，24小时为最佳解释时间。与参比方法比较，喹诺酮类药物尤其是左氧氟沙星的检测结果不理想。此外，研究发现血琼脂和巧克力琼脂可以帮助减少粘液的表达，加快其生长速度。结论：血液MHA可替代MHA，最佳解释时间为24小时。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Antimicrobial Chemotherapy 医学-传染病学

CiteScore

9.20

自引率

5.80%

发文量

423

审稿时长

2-4 weeks

期刊介绍： The Journal publishes articles that further knowledge and advance the science and application of antimicrobial chemotherapy with antibiotics and antifungal, antiviral and antiprotozoal agents. The Journal publishes primarily in human medicine, and articles in veterinary medicine likely to have an impact on global health.