Identification of anti-inflammatory and antimicrobial compounds from leaves and rhizome of Iris pseudacorus collected in Ireland bogland using chemical profiling techniques

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-06-08 DOI:10.1016/j.fitote.2025.106671

Özlem Erol , Shipra Nagar , Mohammed Ali Selo , Ismael Obaidi , John J. Walsh , Helen Sheridan , Young Hae Choi

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Abstract

Iris pseudacorus, a species native to Irish wetlands, was investigated for its antimicrobial and anti-inflammatory potential as part of a broader study on bogland medicinal plants. Methanol extracts from leaves and rhizomes were chemically profiled using NMR, LC-MS, and HPTLC, leading to the identification of three bioactive compounds: syringic acid, luteolin 7-O-β-D-glucoside, and liquiritigenin. HPTLC bioautography revealed that these compounds exhibited moderate antimicrobial activity against Staphylococcus aureus ATCC 29213, with MIC values ranging from 128 to 256 μg/mL. In vitro anti-inflammatory assays using THP-1 macrophages further demonstrated significant inhibition of interleukin-6 (IL-6) production, particularly by liquiritigenin, which showed the strongest bioactivity in both assays. These findings highlight the therapeutic potential of I. pseudacorus and support its traditional use in Irish ethnobotany, offering a promising example of bioactive compound discovery from underexplored wetland flora.

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用化学分析技术鉴定爱尔兰假鸢尾叶和根茎中的抗炎和抗菌化合物。

鸢尾花（Iris pseudacorus）是一种原产于爱尔兰湿地的植物，它具有抗菌和抗炎的潜力，是对bogland药用植物进行更广泛研究的一部分。利用NMR、LC-MS和HPTLC对其甲醇提取物进行化学分析，鉴定出3种生物活性化合物：丁香酸、木犀草素7-O-β- d -葡萄糖苷和甘草素。HPTLC生物图谱显示，这些化合物对金黄色葡萄球菌ATCC 29213具有中等抑菌活性，MIC值在128 ~ 256 μg/mL之间。使用THP-1巨噬细胞进行的体外抗炎实验进一步证明了对白细胞间素-6 （IL-6）产生的显著抑制，特别是利尿原素，在两种实验中都显示出最强的生物活性。这些发现突出了假假蕨的治疗潜力，并支持其在爱尔兰民族植物学中的传统用途，为从未被开发的湿地植物群中发现生物活性化合物提供了一个有希望的例子。

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.