Application of DTT in a Case of Pseudoelevated D-Dimer.

IF 0.7 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Clinical laboratory Pub Date : 2025-06-01 DOI:10.7754/Clin.Lab.2025.250313

Xin Liu, Xiaoli Yang, Zhuoling Li, Chunyan Liu

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引用次数: 0

Abstract

Background: The detection of D-dimer is easily affected by interfering substances, causing a false increase in D-dimer levels and leading to incorrect diagnosis and treatment of diseases in clinical practice. Therefore, correcting interference and obtaining true D-dimer results is crucial.

Methods: Gradient dilution test, parallel test with different detection systems, and detection after sample pretreatment with dithiothreitol (DTT) were used to evaluate and correct interference.

Results: The dilution test indicated the presence of detection interference. The Stago detection system shows that the level of D-dimer had dropped to the normal range. After DTT treatment, the D-dimer level of the sample decreased to 0.18 mg/L, consistent with the Stago detection system.

Conclusions: When a patient's D-dimer levels are abnormally elevated but there are no relevant clinical manifestations, the presence of detection interference should be considered. Preprocessing samples with DTT may be a simple and feasible solution to eliminate interference.

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DTT在假性d -二聚体升高中的应用。

背景：d -二聚体的检测容易受到干扰物质的影响，导致d -二聚体水平的虚假升高，导致临床对疾病的错误诊断和治疗。因此，校正干扰和获得真实的d -二聚体结果是至关重要的。方法：采用梯度稀释试验、不同检测系统平行试验、二硫苏糖醇（DTT）预处理后检测等方法对干扰进行评价和纠正。结果：稀释试验提示存在检测干扰。Stago检测系统显示d -二聚体水平已降至正常范围。DTT处理后，样品d -二聚体水平降至0.18 mg/L，与Stago检测体系一致。结论：当患者d -二聚体水平异常升高但无相关临床表现时，应考虑是否存在检测干扰。用DTT对样品进行预处理可能是一种简单可行的消除干扰的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical laboratory 医学-医学实验技术

CiteScore

1.50

自引率

0.00%

发文量

494

审稿时长

3 months

期刊介绍： Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.