Quantitation of BCAA and BCKA in plasma and patient-centric dried blood microsamples in a clinical setting.

IF 1.8 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2025-06-01 Epub Date: 2025-06-10 DOI:10.1080/17576180.2025.2515008

Brendan Tierney, Pirinka Georgiev Tuttle, F Isik Karahanoglu, Christian Russel Reyes, Yalei Chen, Matthew Blatnik, Savon Vigil, Matthew Ziegenfelder, Mar Santamaria, Denise E Chou, Bunmi Williams, Anna Gao, Briana Peterson, Bhavna Adhin, Xuemei Cai

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引用次数: 0

Abstract

Aim: This study assessed the agreement and bias of three dried blood patient-centric microsampling (PCS) devices (paper DBS, Mitra, and Tasso-M20) for the quantitation of branched chain amino acids (BCAAs) and ketoacids (BCKAs) compared to venous plasma samples.

Materials & methods: Concentrations of BCAAs and BCKAs were measured in samples from generally healthy participants with a validated assay for venous plasma adapted for use with dried blood from the PCS devices. Participants were also asked to respond to a device usability questionnaire about their experience with the devices.

Results: The correlations between BCAA/BCKA concentration values in PCS devices vs venipuncture samples were strong to excellent for all six analytes, across all three PCS devices. The surrogate analyte approach facilitated method transfer from plasma to dried blood microsampling devices. Overall, participants reported that they enjoyed using the devices compared to traditional venipuncture and would be willing to use them again, on their own at-home.

Conclusion: These study results demonstrate the feasibility of quantitating BCAAs and BCKAs reliably with PCS devices, with high acceptability from study participants.

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血浆和以患者为中心的干血微样本中BCAA和BCKA的定量研究

目的：本研究评估了三种以患者为中心的干血微采样（PCS）设备（纸制DBS、Mitra和Tasso-M20）与静脉血浆样品相比用于支链氨基酸（BCAAs）和酮酸（BCKAs）定量的一致性和偏置性。材料和方法：在一般健康参与者的样本中测量BCAAs和BCKAs的浓度，采用经过验证的静脉血浆测定法，适用于PCS设备的干血。参与者还被要求回答一份关于他们使用设备体验的设备可用性问卷。结果：PCS设备中BCAA/BCKA浓度值与静脉穿刺样品之间的相关性在所有三种PCS设备中，所有六种分析物都具有很强的相关性。替代分析物方法促进了方法从血浆转移到干血微采样装置。总的来说，参与者报告说，与传统的静脉穿刺相比，他们喜欢使用这些设备，并且愿意在自己家里再次使用它们。结论：本研究结果证明了用PCS装置定量BCAAs和BCKAs的可行性，研究参与者的接受度高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.