Diversity of carbapenemase genes among carbapenem resistant Klebsiella pneumoniae in a tertiary care hospital in Bangkok, Thailand.

IF 1.3 4区医学 Q4 IMMUNOLOGY

Acta microbiologica et immunologica Hungarica Pub Date : 2025-06-10 Print Date: 2025-06-20 DOI:10.1556/030.2025.02599

Anchalee Homkaew, Thanwa Wongsuk, Siriphan Boonsilp, Jutturong Ckumdee, Padungsri Dubbs, Prasit Palittapongarnpim

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Abstract

Multidrug resistant (MDR) gram-negative bacilli associated with hospital-acquired infections are commonly resistant to carbapenems. Klebsiella pneumoniae is a common MDR Enterobacterales in Thailand. In this study, we investigated the distribution of five carbapenemase genes (blaNDM, blaOXA-48, blaIMP, blaVIM, and blaKPC) among 62 carbapenem resistant K. pneumoniae (CRKP) collected in 2022 from patients admitted to a tertiary care hospital in Bangkok. The frequencies of isolates carrying a single carbapenamase gene were 39% for blaOXA-48 and 19% for blaNDM. Interestingly the frequency of the carriers of both genes was as high as 29% and none of the isolates carried blaKPC, commonly reported elsewhere. The studied genes were not identified in 7 isolates (11%). CRKP carrying blaNDM was more frequently identified in medical wards, associated with higher mortality rate and 100% resistant to ceftazidime/avibactam while the one carrying only blaOXA-48 was 92% susceptible to ceftazidime/avibactam. This study confirms the advantage of molecular methods for differentiating between mechanisms of carbapenem resistance in K. pneumoniae.

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泰国曼谷一家三级医院耐碳青霉烯类肺炎克雷伯菌碳青霉烯类酶基因多样性

与医院获得性感染相关的多重耐药（MDR）革兰氏阴性杆菌通常对碳青霉烯类耐药。肺炎克雷伯菌是泰国常见的耐多药耐药肠杆菌。在这项研究中，我们调查了5种碳青霉烯酶基因（blaNDM、blaOXA-48、blaIMP、blaVIM和blaKPC）在2022年从曼谷一家三级医院收治的62例碳青霉烯类耐药肺炎克雷伯菌（CRKP）中的分布。blaOXA-48和blaNDM分别有39%和19%的分离株携带单个碳青霉烯酶基因。有趣的是，这两种基因的携带者频率高达29%，而且没有一种分离物携带blaKPC，这在其他地方普遍报道。7株（11%）未检出所研究的基因。携带blaNDM的CRKP在病房更常见，死亡率更高，对头孢他啶/阿维巴坦100%耐药，而仅携带blaOXA-48的CRKP对头孢他啶/阿维巴坦92%易感。本研究证实了分子方法区分肺炎克雷伯菌碳青霉烯类耐药机制的优势。

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来源期刊

Acta microbiologica et immunologica Hungarica IMMUNOLOGY-MICROBIOLOGY

CiteScore

2.30

自引率

13.30%

发文量

审稿时长

>12 weeks

期刊介绍： AMIH is devoted to the publication of research in all fields of medical microbiology (bacteriology, virology, parasitology, mycology); immunology of infectious diseases and study of the microbiome related to human diseases.