{"title":"Characterization of DnaB–DnaG Interaction in M. tuberculosis Using Small-Angle X-ray Scattering-Based Dissociation Assay","authors":"Dayan A, Ilic S, Akabayov B","doi":"10.1002/cbic.202500289","DOIUrl":null,"url":null,"abstract":"<p>The complex interactions between helicase and primase, two key components of the replisome involved in DNA replication in <i>Mycobacterium tuberculosis</i> are studied. Utilizing purified, complementary domains of these proteins, a surface plasmon resonance (SPR) analysis and a cross-linking assay to characterize their binding dynamics are employed. The SPR analysis reveals a binding dissociation constant of 0.21 ± 0.08 μM, and the cross-linking assay suggests the possible formation of a heterodimer species. Importantly, a small-angle X-ray scattering dissociation assay to study the dynamic interactions between the proteins in solution is utilized. The findings provide new opportunities for targeted therapeutic strategies aimed at DNA replication in <i>M. tuberculosis</i> by revealing the structural interplay between helicase and primase.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 14","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbic.202500289","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ChemBioChem","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cbic.202500289","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The complex interactions between helicase and primase, two key components of the replisome involved in DNA replication in Mycobacterium tuberculosis are studied. Utilizing purified, complementary domains of these proteins, a surface plasmon resonance (SPR) analysis and a cross-linking assay to characterize their binding dynamics are employed. The SPR analysis reveals a binding dissociation constant of 0.21 ± 0.08 μM, and the cross-linking assay suggests the possible formation of a heterodimer species. Importantly, a small-angle X-ray scattering dissociation assay to study the dynamic interactions between the proteins in solution is utilized. The findings provide new opportunities for targeted therapeutic strategies aimed at DNA replication in M. tuberculosis by revealing the structural interplay between helicase and primase.
期刊介绍：
ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
