Real-Time Intracellular Monitoring of miRNA Dynamics during Induced Pluripotent Stem Cell Neuronal Differentiation via Plasmon-Enhanced Nanobiosensing.

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-07-02 Epub Date: 2025-06-10 DOI:10.1021/acs.nanolett.5c01840

Yannan Hou, Meizi Chen, Letao Yang, Ki-Bum Lee

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Abstract

Induced pluripotent stem cells (iPSCs) offer immense potential for treating central nervous system (CNS) disorders and injuries. However, the lack of highly sensitive, selective, and noninvasive biosensors for real-time monitoring of iPSC neuronal differentiation remains a critical barrier. In this work, we introduce a gold nanorod-based metal-enhanced molecular beacon (MEMB) nanobiosensor for the noninvasive, real-time detection of intracellular miRNA-124, a key biomarker for neuronal differentiation in human iPSC-derived neural stem cells. Designed through finite-difference time-domain (FDTD) simulations and experimentally validated for optimized localized surface plasmon resonance (LSPR) properties, MEMB nanobiosensors achieved picomolar-level sensitivity and single-mismatch selectivity toward miRNA-124 detection, along with great biocompatibility demonstrated by live-cell assays. Collectively, the MEMB platform provides a robust analytical tool for in-depth investigations of molecular and genetic regulatory networks during iPSC neuronal differentiation in a nondestructive manner, paving the way toward safer, more efficient, and better-characterized iPSC-derived cell therapies for CNS diseases and injuries.

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利用等离子体增强纳米生物传感技术实时监测诱导多能干细胞神经元分化过程中的miRNA动态。

诱导多能干细胞（iPSCs）在治疗中枢神经系统（CNS）疾病和损伤方面具有巨大潜力。然而，缺乏用于实时监测iPSC神经元分化的高灵敏度、选择性和非侵入性生物传感器仍然是一个关键障碍。在这项工作中，我们引入了一种基于金纳米棒的金属增强分子信标（MEMB）纳米生物传感器，用于无创、实时检测细胞内miRNA-124， miRNA-124是人类ipsc来源的神经干细胞神经元分化的关键生物标志物。MEMB纳米生物传感器通过时域有限差分（FDTD）模拟设计，并通过实验验证了优化的局部表面等离子体共振（LSPR）特性，实现了对miRNA-124检测的皮摩尔级灵敏度和单错配选择性，并通过活细胞实验证明了其良好的生物相容性。总的来说，MEMB平台提供了一个强大的分析工具，以非破坏性的方式深入研究iPSC神经元分化过程中的分子和遗传调控网络，为更安全、更有效和更好地表征iPSC衍生细胞治疗中枢神经系统疾病和损伤铺平了道路。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.