Polysaccharide from natural Ophiocordyceps sinensis alleviated dextran sulfate sodium-induced colitis through modulating sIgA formation and gut microbiota

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-06-12 DOI:10.1016/j.jff.2025.106921

Shuping Chen , Junqiao Wang , Qiuyue Fang , Nan Dong , Steve W. Cui , Shaoping Nie

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Abstract

Polysaccharide from natural Ophiocordyceps sinensis (NCSP-50) significantly modulated the gut microbiota composition and elevated the levels of sIgA in dextran sodium sulfate (DSS)-induced colitis mice. However, the in-depth mechanism remains unknown. Therefore, this study aimed to investigate how NCSP-50 influenced sIgA formation and the role of gut microbiota in colitis mice. Results showed that administration of NCSP-50 dramatically improved the expressions of IgA, pIgR and AID, the key factors of sIgA formation in colitis mice. NCSP-50 significantly decreased the quantity of dendritic cells and mRNA expression of tlr-2, tlr-4 and tlr-6, april and raldh-1, while improved the expression of vapc2, raldh-2 and iNOS, indicating the involvement in T-cell independent pathway. NCSP-50 reduced the amount of CD4⁺ T cells, and adjusted the expression of nuclear transcription factors of T cells subsets and the release of IL-13, indicating the involvement in T-cell dependent pathway. In addition, fecal microbiota transplantation revealed that gut microbiota from mice administrated with NCSP-50 obviously alleviated colitis-related symptoms and increased the concentration of sIgA, whereas antibiotic cocktails treatments abolished those effects. In conclusion, NCSP-50 attenuated DSS-induced colitis through intestinal microbiota and promoting the T-cell dependent and independent sIgA secretion influenced by gut microbiota.

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天然冬虫夏草多糖通过调节sIgA的形成和肠道菌群减轻葡聚糖硫酸钠诱导的结肠炎

天然冬虫夏草多糖（NCSP-50）可显著调节右旋糖酐硫酸钠（DSS）诱导的结肠炎小鼠肠道菌群组成，提高sIgA水平。然而，其深层机制尚不清楚。因此，本研究旨在探讨NCSP-50对结肠炎小鼠sIgA形成的影响及肠道菌群的作用。结果表明，NCSP-50可显著提高结肠炎小鼠sIgA形成的关键因子IgA、pIgR和AID的表达。NCSP-50显著降低树突状细胞数量及tlr-2、tlr-4、tlr-6、april和raldh-1 mRNA表达，提高vapc2、raldh-2和iNOS表达，提示其参与t细胞非依赖性通路。NCSP-50可降低CD4+ T细胞的数量，调节T细胞亚群核转录因子的表达和IL-13的释放，提示参与T细胞依赖通路。此外，粪便微生物群移植显示，给予NCSP-50的小鼠肠道微生物群明显缓解了结肠炎相关症状，并增加了sIgA浓度，而抗生素鸡尾酒治疗则消除了这些作用。综上所述，NCSP-50通过肠道菌群减轻dss诱导的结肠炎，促进受肠道菌群影响的t细胞依赖性和独立sIgA分泌。

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.