Molecular study of candiduria in pediatric patients in in relation to biofilm formation and fluconazole tolerance

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-06-07 DOI:10.1016/j.diagmicrobio.2025.116943

Rasha H. ElKenawy , Maysaa El Sayed Zaki , Sara Ghaleb Essa , Noha Mostafa Mahmoud , Ahmed Hazem El-Nagdy , Mona Abdellatif , Mayada Sabry Zeid

{"title":"Molecular study of candiduria in pediatric patients in in relation to biofilm formation and fluconazole tolerance","authors":"Rasha H. ElKenawy , Maysaa El Sayed Zaki , Sara Ghaleb Essa , Noha Mostafa Mahmoud , Ahmed Hazem El-Nagdy , Mona Abdellatif , Mayada Sabry Zeid","doi":"10.1016/j.diagmicrobio.2025.116943","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>There are limited studies about the presence of <em>Candida</em> species <em>(Candida</em> spp.<em>)</em> and ABC genotypes in hospital acquired urinary tract infection (UTI) in children.</div></div><div><h3>Aim</h3><div>the study aimed at identification of <em>Candida spp.</em> associated with candiduria in children, detection of fluconazole tolerance and biofilm formation and genotyping of <em>C. albicans</em>.</div></div><div><h3>Method</h3><div>The study was conducted on pediatric patients with hospital-acquired UTI. A urine sample was cultured on the agar CHROM <em>Candida</em>. Molecular identification of <em>Candida spp.</em> was performed by nested polymerase chain reaction (PCR) and specific genotyping of C. albicans was performed by PCR. Biofilm production and fluconazole tolerance were tested for the isolates.</div></div><div><h3>Results</h3><div>97 children were included. Urinary catheters were inserted reported in 69.1 % of the children and previous antibiotic intake was reported in 34 % of them. The identified species were <em>C. albicans</em> (66 %) followed by <em>Nakaseomyces glabrat</em>a (19.6 %), <em>Candida tropicalis</em> (11.3 %) and <em>Candida kruzei</em> (3.1 %). The ability of biofilm formation was 100 %<em>.</em> The <em>Pichia kudriavzevii</em> had a highest significant rate of fluconazole tolerance (66.7%), (P=0.045). Resistance to fluconazole was observed significantly with <em>C. tropicalis</em> (63.6%), (P=0.033). The common genotypes of <em>C. albicans</em> were A (60.9%) and C (39.1%). There was significant capacity of <em>C. albicans</em> genotype C to form strong biofilm (P<0.001).</div></div><div><h3>Conclusion</h3><div>The study found that <em>C. albicans</em> remains the most prevalent species in candiduria. The ability of <em>Candida spp.</em> to form biofilms was 100% among the isolates. Strong biofilm formation being significantly associated with increased fluconazole resistance and <em>C. albicans</em> genotype C.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 2","pages":"Article 116943"},"PeriodicalIF":2.1000,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diagnostic microbiology and infectious disease","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0732889325002664","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Background

There are limited studies about the presence of Candida species (Candida spp.) and ABC genotypes in hospital acquired urinary tract infection (UTI) in children.

Aim

the study aimed at identification of Candida spp. associated with candiduria in children, detection of fluconazole tolerance and biofilm formation and genotyping of C. albicans.

Method

The study was conducted on pediatric patients with hospital-acquired UTI. A urine sample was cultured on the agar CHROM Candida. Molecular identification of Candida spp. was performed by nested polymerase chain reaction (PCR) and specific genotyping of C. albicans was performed by PCR. Biofilm production and fluconazole tolerance were tested for the isolates.

Results

97 children were included. Urinary catheters were inserted reported in 69.1 % of the children and previous antibiotic intake was reported in 34 % of them. The identified species were C. albicans (66 %) followed by Nakaseomyces glabrata (19.6 %), Candida tropicalis (11.3 %) and Candida kruzei (3.1 %). The ability of biofilm formation was 100 %. The Pichia kudriavzevii had a highest significant rate of fluconazole tolerance (66.7%), (P=0.045). Resistance to fluconazole was observed significantly with C. tropicalis (63.6%), (P=0.033). The common genotypes of C. albicans were A (60.9%) and C (39.1%). There was significant capacity of C. albicans genotype C to form strong biofilm (P<0.001).

Conclusion

The study found that C. albicans remains the most prevalent species in candiduria. The ability of Candida spp. to form biofilms was 100% among the isolates. Strong biofilm formation being significantly associated with increased fluconazole resistance and C. albicans genotype C.

查看原文本刊更多论文

儿童患者念珠菌与生物膜形成和氟康唑耐受性的分子研究

背景关于念珠菌种类（念珠菌属）和ABC基因型在儿童医院获得性尿路感染（UTI）中的存在的研究有限。本研究旨在鉴定儿童念珠菌相关念珠菌，检测白念珠菌的氟康唑耐受性和生物膜形成情况，并进行基因分型。方法以医院获得性尿路感染患儿为研究对象。尿液样本在琼脂CHROM念珠菌上培养。采用巢式聚合酶链反应（巢式PCR）对念珠菌进行分子鉴定，对白色念珠菌进行特异性基因分型。对分离菌株进行了生物膜生成和氟康唑耐受性试验。结果纳入97例患儿。据报道，69.1%的儿童插入了导尿管，其中34%的儿童曾服用过抗生素。鉴定的菌种以白色念珠菌（66%）次之，其次为光秃中丝酵母菌（19.6%）、热带念珠菌（11.3%）和克鲁泽念珠菌（3.1%）。生物膜的形成率为100%。毕赤酵母对氟康唑的耐受性最高（66.7%）（P=0.045）。热带恙螨对氟康唑的耐药率为63.6% (P=0.033)；白色念珠菌常见基因型为A型（60.9%）和C型（39.1%）。基因型C的白色念珠菌形成强生物膜的能力显著（P<0.001）。结论研究发现白色念珠菌仍是念珠菌中最常见的菌种。念珠菌在分离株中形成生物膜的能力为100%。强生物膜形成与氟康唑耐药性和白色念珠菌基因型增加显著相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.