Diverse Acinetobacter in retail meat: a hidden vector of novel species and antimicrobial resistance genes, including plasmid-borne blaOXA-58, mcr-4.3 and tet(X3)

IF 5.2 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

International journal of food microbiology Pub Date : 2025-06-10 DOI:10.1016/j.ijfoodmicro.2025.111313

Alba Puente , José F. Cobo-Díaz , Márcia Oliveira , Rebeca Cordero-García , Filipa Grosso , Francesco Asnicar , Nicola Segata , Yara El dessouky , Mercedes López , Miguel Prieto , Luísa Peixe , Avelino Alvarez-Ordóñez

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引用次数: 0

Abstract

Acinetobacter species, particularly Acinetobacter baumannii, are recognized pathogens in clinical settings, yet their presence in food systems, including fresh meat remains underexplored. This comprehensive study investigated the prevalence, diversity, concentration, and antimicrobial resistance of Acinetobacter spp. in 100 fresh meat samples from diverse animal sources across various packaging conditions.

Acinetobacter isolates were initially characterized by MALDI-TOF MS, with comprehensive genomic characterization through whole-genome sequencing (WGS) of 116 representative isolates. Taxonomic refinement was performed using GTDB-Tk, core-genome, rpoB gene and Average Nucleotide Identity (ANI) phylogenomic approaches. Antimicrobial resistance genes (ARGs), and their plasmidic locations, were identified, and antimicrobial susceptibility profiles were determined for 33 A. baumannii isolates.

Acinetobacter spp. were detected in 74 % of samples, with turkey meat showing the highest occurrence. The counts of this bacterium ranged from < 0.23 to 3.13 log₁₀ CFU/g. A total of 20 know species and 2 putative novel Acinetobacter species were identified by genomic analysis. Moreover, 16 novel A. baumannii sequence types (STs) were identified. ARG profiling revealed a complex resistome, including plasmid-located ARGs spanning multiple antibiotic classes. Critical findings include the presence of plasmid-borne bla_OXA-58, mcr-4.3, and tet(X3) genes.

This study expands our understanding of Acinetobacter spp. diversity and reveals fresh meat as a significant vector for this genus, including species associated with human infections. Moreover, the detection of diverse resistance genes, including some associated with plasmids and conferring resistance to critically important antibiotics, underscores the potential public health implications of meat as a transmission pathway for these bacteria.

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零售肉类中多种不动杆菌：新物种和耐药基因的隐藏载体，包括质粒携带的blaOXA-58、mcr-4.3和tet（X3）

不动杆菌种类，特别是鲍曼不动杆菌，是临床环境中公认的病原体，但它们在食品系统（包括鲜肉）中的存在仍未得到充分探索。本综合研究调查了不同包装条件下来自不同动物来源的100个鲜肉样品中不动杆菌的流行率、多样性、浓度和抗菌素耐药性。采用MALDI-TOF MS对不动杆菌分离株进行了初步鉴定，并通过全基因组测序（WGS）对116株代表性分离株进行了全面的基因组鉴定。采用GTDB-Tk、核心基因组、rpoB基因和平均核苷酸识别（ANI）系统基因组方法进行分类改进。鉴定了33株鲍曼不动杆菌的耐药基因（ARGs）及其质粒位置，并测定了其药敏谱。在74%的样品中检测到不动杆菌，其中火鸡肉的发生率最高。这种细菌的计数范围从<；0.23 ~ 3.13 log10 CFU/g。通过基因组分析共鉴定出20种已知不动杆菌和2种推定的新不动杆菌。此外，还鉴定出16种新的鲍曼不动杆菌序列类型（STs）。ARG分析揭示了一个复杂的抵抗组，包括质粒定位的ARG，跨越多种抗生素类别。关键发现包括质粒携带的blaOXA-58、mcr-4.3和tet（X3）基因的存在。这项研究扩大了我们对不动杆菌多样性的理解，并揭示了鲜肉是该属的重要载体，包括与人类感染有关的物种。此外，多种耐药基因的检测，包括一些与质粒相关的基因和对至关重要的抗生素产生耐药性的基因，强调了肉类作为这些细菌传播途径的潜在公共卫生影响。

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来源期刊

International journal of food microbiology 工程技术-食品科技

CiteScore

10.40

自引率

5.60%

发文量

322

审稿时长

65 days

期刊介绍： The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.