Transcriptome and proteome analyses reveal the virulence of the Vibrio alginolyticus effector gene vopR.

IF 3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular omics Pub Date : 2025-06-10 DOI:10.1039/d5mo00010f

Fan Da, Shuanghu Cai, Liangliang Xu, Shixi Chen, Bin Li, Min Tao

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Abstract

Vibrio alginolyticus is an opportunistic infectious pathogen, and its pathogenicity is related to various virulence factors, with the type III secretion system (T3SS) being one of the important systems for secreting virulence factors. vopR is one of the effector genes of V. alginolyticus T3SS1. To investigate its pathogenicity, this study constructed an overexpression vector to express vopR in host cells, using the empty plasmid as a control, and analyzed its impact on the mRNA and protein expression levels of host cells. Transcriptomic analysis revealed that overexpression of vopR led to the upregulation of 410 genes and the downregulation of 207 genes. Proteomic analysis showed that 126 proteins were upregulated, and 518 proteins were downregulated. GO enrichment analysis indicated that the differential genes were significantly enriched in various biological processes such as cellular processes, metabolic processes, and biological regulation, as well as in cellular components like cell parts and membranes, and molecular functions such as binding and catalytic activity. KEGG enrichment analysis demonstrated that the differential genes were mainly enriched in metabolic pathways, cancer-related pathways, and the MAPK signaling pathway. The combined analysis of the transcriptome and proteome screened out 144 overlapping differentially expressed genes, with 60 being upregulated and 54 being downregulated. These results suggest that vopR has a significant impact on the cytoskeleton, metabolism, and immune regulation of host cells during the pathogenic process of V. alginolyticus. This study provides a theoretical basis for a deeper understanding of the pathogenic mechanism of V. alginolyticus.

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转录组和蛋白质组分析揭示了溶藻弧菌效应基因vopR的毒力。

溶藻弧菌是一种机会性感染性病原体，其致病性与多种毒力因子有关，其中III型分泌系统（T3SS）是分泌毒力因子的重要系统之一。vopR是溶藻弧菌T3SS1的效应基因之一。为了研究其致病性，本研究构建了vopR在宿主细胞中的过表达载体，以空质粒为对照，分析其对宿主细胞mRNA和蛋白表达水平的影响。转录组学分析显示，vopR过表达导致410个基因上调，207个基因下调。蛋白质组学分析显示126个蛋白表达上调，518个蛋白表达下调。氧化石墨烯富集分析表明，差异基因在细胞过程、代谢过程、生物调控等多种生物过程中，以及在细胞部分、细胞膜等细胞组分和结合、催化活性等分子功能中均显著富集。KEGG富集分析表明，差异基因主要富集于代谢途径、癌症相关途径和MAPK信号通路。转录组和蛋白质组的联合分析筛选出144个重叠的差异表达基因，其中60个表达上调，54个表达下调。这些结果表明，在溶藻弧菌的致病过程中，vopR对宿主细胞的细胞骨架、代谢和免疫调节有显著影响。本研究为深入了解溶藻弧菌的致病机制提供了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

5.40

自引率

3.40%

发文量

期刊介绍： Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.