A novel small droplets freezing method for human sperm cryopreservation: maximizing utilization of limited samples.

IF 3.2 3区医学 Q2 GENETICS & HEREDITY

Journal of Assisted Reproduction and Genetics Pub Date : 2025-06-10 DOI:10.1007/s10815-025-03532-3

Yael Harir, Rona Halevy Amiran, Miriam Almagor, Sheila Fieldust, Zeev Shoham, Yuval Or

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引用次数: 0

Abstract

Purpose: The study aims to evaluate and compare sperm progressive motility before and after cryopreservation using the conventional slow freezing technique versus a novel small droplets freezing method. This research seeks to determine the optimal freezing technique, particularly for preserving unique and irreplaceable sperm samples.

Methods: Seventy-five sperm samples were divided and cryopreserved using two techniques: (1) the classic slow freezing method and (2) the small droplets freezing method. After thawing, sperm progressive motility was assessed and compared between the two methods.

Results: No significant difference in sperm progressive motility was observed between the two techniques. The mean post-thaw progressive motility was 22.9% for the classical method and 23.64% for the small droplets method (p = 0.74), indicating comparable outcomes.

Conclusion: Cryopreservation in small droplets maintains sperm progressive motility similarly to conventional slow freezing. This technique allows for more efficient utilization of limited sperm samples, making it particularly valuable for posthumous sperm retrieval, fertility preservation in oncological patients, and cases where sperm availability is extremely limited.

查看原文本刊更多论文

一种用于人类精子冷冻保存的小液滴冷冻新方法：最大限度地利用有限的样本。

目的：本研究旨在评估和比较传统慢速冷冻技术与新型小液滴冷冻技术冷冻前后精子的进展活力。本研究旨在确定最佳冷冻技术，特别是保存独特和不可替代的精子样本。方法：采用经典慢速冷冻法和小液滴冷冻法对75份精子样本进行分离和冷冻保存。解冻后，评估和比较两种方法的精子进行性运动。结果：两种方法在精子进行性运动方面无显著差异。经典法和小液滴法解冻后的平均渐进运动率分别为22.9%和23.64% (p = 0.74)，结果具有可比性。结论：小液滴低温保存与常规慢速冷冻一样，能维持精子的进行性运动。该技术允许更有效地利用有限的精子样本，使其在死后精子提取，肿瘤患者的生育能力保存以及精子供应极其有限的情况下特别有价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Assisted Reproduction and Genetics 医学-妇产科学

CiteScore

5.70

自引率

9.70%

发文量

286

审稿时长

1 months

期刊介绍： The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species. The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.