Cell-based evaluation of anti-inflammatory activity from the combination of natural compounds in LPS-stimulated U937 monocytes.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cytotechnology Pub Date : 2025-06-01 Epub Date: 2025-06-07 DOI:10.1007/s10616-025-00788-6

Maria Giovanna Rizzo, Cristiana Roberta Multisanti, Caterina Faggio, Federica Impellitteri

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引用次数: 0

Abstract

The present study aims to examine the potential effects of a novel coupling of natural-derived compounds modulating inflammatory responses. Their effects were assessed in LPS-inflamed U937 pro-monocytes. Monocytes were exposed to incremental concentrations of citric acid (2-hydroxy-1, 2, 3-propanetricarboxylic acid) and quercetin (3, 3', 4', 5, 7-pentahydroxyflavone), administered individually and in combination. Evaluation of cell viability was conducted via the MTT assay; nitric oxide (NO) production was assessed using the Griess assay; the expression levels of key pro-inflammatory markers (IL-1β, IL-6, TNF-α, and COX-2) were determined through quantitative real-time PCR (qRT-PCR). The results indicate that the two combined substances modulate the pathways of anti-inflammatory cytokines in pro-monocytic cells. Collectively, our data indicated that the synergistic activity of quercetin and citric acid has the potential to be developed as a novel therapeutic agent for inflammatory-related diseases, also opening new avenues for developing natural strategies in pathologies related to inflammatory processes treatment.

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lps刺激的U937单核细胞中天然化合物组合的抗炎活性基于细胞的评估

本研究旨在研究一种新型天然衍生化合物偶联调节炎症反应的潜在作用。在lps炎症的U937前单核细胞中评估它们的作用。单核细胞暴露于增加浓度的柠檬酸（2-羟基- 1,2,3 -丙三羧酸）和槲皮素（3,3 ',4',5,7 -五羟基黄酮），单独或联合施用。MTT法测定细胞活力；采用Griess法评估一氧化氮（NO）的产生；采用实时荧光定量PCR （qRT-PCR）检测各组关键促炎标志物IL-1β、IL-6、TNF-α、COX-2的表达水平。结果表明，两种联合物质可调节单核细胞前抗炎细胞因子的通路。总的来说，我们的数据表明，槲皮素和柠檬酸的协同活性有可能被开发为炎症相关疾病的新型治疗剂，也为开发炎症过程治疗相关病理的自然策略开辟了新的途径。

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来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.