Phylogenetic Analysis of SOD Gene Isolated from Indian Variety of Mud Crabs: Scylla serrata and Scylla olivacea.

IF 1.9 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current protein & peptide science Pub Date : 2025-06-05 DOI:10.2174/0113892037375925250519112911

Manu Asthana, Javed Masood Khan, Chittibabu Shanthi

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Abstract

Aim: Our research aimed to isolate and sequence the SOD gene from the genomic DNA of Scylla serrata and Scylla olivacea and to study its phylogeny.

Background: In crustaceans, superoxide dismutase (SOD) serves as the first line of defense against stress. Extracellular Cu/Zn-SOD has been demonstrated in several investigations involving crustaceans. Crustaceans do not have a distinct immune system. They entirely depend on the innate immune system triggered when they come in contact with any pathogen.

Method: Partial SOD gene was isolated from the genomic DNA of S. serrata and S. olivacea through polymerase chain reaction.

Result: We successfully isolated partial SOD genes of 942bp and 957bp from S. serrata and S. olivacea, respectively. The sequences were submitted to the NCBI GenBank database.

Discussion: The phylogenetic study suggests their clustering with the genus Scylla species. Investigating the SOD gene sequences across diverse crustacean lineages can reveal profound insights into their evolutionary history and the intricate relationships among species concerning their SOD development.

Conclusion: This research holds the potential to enhance our understanding of the evolutionary adaptations that have shaped these organisms.

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印度泥蟹品种Scylla serrata和Scylla olivacea SOD基因分离的系统发育分析。

目的：从锯齿山Scylla serrata和橄榄山Scylla olivacea的基因组DNA中分离SOD基因并对其进行测序，研究其系统发育。背景：在甲壳类动物中，超氧化物歧化酶（SOD）是抵御应激的第一道防线。细胞外Cu/Zn-SOD已在一些涉及甲壳类动物的研究中得到证实。甲壳类动物没有独特的免疫系统。它们完全依赖于先天免疫系统，当它们接触到任何病原体时就会被触发。方法：采用聚合酶链反应，从蛇蛇和橄榄山蛇的基因组DNA中分离出部分SOD基因。结果：成功分离到serrata s.s serrata和olivacea s.s的SOD基因片段，分别为942bp和957bp。序列提交至NCBI GenBank数据库。讨论：系统发育研究表明它们属于Scylla属。研究不同甲壳类动物的SOD基因序列，可以深入了解甲壳类动物的进化史和物种间SOD发育的复杂关系。结论：这项研究有可能增强我们对形成这些生物的进化适应的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protein & peptide science 生物-生化与分子生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Current Protein & Peptide Science publishes full-length/mini review articles on specific aspects involving proteins, peptides, and interactions between the enzymes, the binding interactions of hormones and their receptors; the properties of transcription factors and other molecules that regulate gene expression; the reactions leading to the immune response; the process of signal transduction; the structure and function of proteins involved in the cytoskeleton and molecular motors; the properties of membrane channels and transporters; and the generation and storage of metabolic energy. In addition, reviews of experimental studies of protein folding and design are given special emphasis. Manuscripts submitted to Current Protein and Peptide Science should cover a field by discussing research from the leading laboratories in a field and should pose questions for future studies. Original papers, research articles and letter articles/short communications are not considered for publication in Current Protein & Peptide Science.