Optimizing dietary crude protein, branched-chain amino acids, and energy levels for broiler performance using a Box-Behnken design.

IF 6.1 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Nutrition Pub Date : 2025-04-25 eCollection Date: 2025-06-01 DOI:10.1016/j.aninu.2025.02.006

Sosthene Musigwa, Pierre Cozannet, Mingan Choct, Shu-Biao Wu

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Abstract

This study aimed to investigate the limitations and interactive effects of dietary crude protein (CP; 15%, 17% and 19%), excess branched-chain amino acid (BCAA) inclusions (0%, 20% and 40%), and net energy (NE; 9.0, 9.7 and 10.4 MJ/kg) on performance and nutrient utilization for Cobb 500 mix-sex broilers, using a Box-Behnken design. The study consisted of 2 experiments: Exp. 1 involved 1092 chickens, and their performance was measured from d 19 to 35, and Exp. 2 employed 156 birds for NE measurements from d 25 to 28. Both experiments used the same diets (n = 13), each replicated 7 times for Exp. 1 and 6 times for Exp. 2. On d 35, 4 birds per pen (2 males and 2 females) were sampled to collect ileal digesta and weigh carcass parts. Feed intake (FI), NE intake (NEi), weight gain (WG), feed conversion ratio (FCR) and breast yield were affected by CP × NE (P < 0.001) and CP × BCAA (P = 0.041). Diluting NE in the reduced CP (RP)-diets led to a higher FI (P < 0.001) and breast yield (P < 0.001) than high NE, but the NE levels had no effect (P > 0.05) on FI and breast yield in high CP (HCP)-diets. Breast yield increased (P = 0.041) with BCAA in HCP-diets, whereas in the RP-diets, the yield lowered (P = 0.041) with increased BCAA. A similar trend was observed with fat content: in HCP-diets, fat content increased (P = 0.007) with BCAA, while in RP-diets, fat content decreased (P = 0.007) with higher BCAA levels. In addition, increasing BCAA in the RP-diets decreased (P < 0.001) FI, NEi, WG and increased FCR (P = 0.001) than low BCAA. However, BCAA levels had no effect (P > 0.05) on these measurements in HCP-diets. High NE increased (P < 0.001) NEi and decreased (P < 0.001) FCR compared to low NE in the HCP-diets. However, the NE effect on both measurements did not differ (P > 0.05) in RP-diets. These results indicate that increasing BCAA levels beyond the recommended amounts in RP-diets impairs energy utilization, leading to poor performance.

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采用Box-Behnken设计优化饲粮粗蛋白质、支链氨基酸和能量水平，提高肉鸡生产性能。

本研究旨在探讨饲粮粗蛋白质(CP；15%、17%和19%)，多余支链氨基酸（BCAA）内含物（0%、20%和40%）和净能量(NE；9.0、9.7和10.4 MJ/kg)对Cobb 500混合性肉鸡生产性能和养分利用的影响，采用Box-Behnken设计。试验1选取1092只鸡，在第19 ~ 35天测定其生产性能；试验2选取156只鸡，在第25 ~ 28天测定其NE。两个试验均采用相同的饲粮（n = 13），试验1重复7次，试验2重复6次。第35天，每栏4只（雌雄各2只）采集回肠食糜并称重胴体部位。CP × NE （P < 0.001）和CP × BCAA （P = 0.041）对采食量（FI）、NE采食量（NEi）、增重（WG）、饲料系数（FCR）和乳腺产量均有显著影响。在低粗蛋白质（RP）饲粮中稀释NE比高粗蛋白质饲粮中增加FI （P < 0.001）和乳房产量（P < 0.001），但NE水平对高粗蛋白质（HCP）饲粮中的FI和乳房产量没有影响（P < 0.05）。在hcp饲粮中，随着BCAA的增加，乳房产量增加（P = 0.041），而在rp饲粮中，随着BCAA的增加，乳房产量降低（P = 0.041）。脂肪含量也有类似的变化趋势：在hcp饲粮中，脂肪含量随着BCAA的增加而增加（P = 0.007），而在rp饲粮中，脂肪含量随着BCAA的增加而降低（P = 0.007）。此外，与低BCAA相比，增加rp -饲粮中的BCAA降低了FI、NEi、WG (P < 0.001)，增加了FCR （P = 0.001）。然而，在hcp日粮中，BCAA水平对这些测量没有影响（P < 0.05）。与低NE相比，高NE增加了NEi (P < 0.001)，降低了FCR （P < 0.001）。然而，在rp日粮中，NE对两项测量的影响没有差异（P < 0.05）。这些结果表明，rp日粮中超过推荐量的BCAA水平会损害能量利用，导致性能下降。

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来源期刊

Animal Nutrition Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

7.40

自引率

3.20%

发文量

172

审稿时长

12 weeks

期刊介绍： Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to nutrition, and more applied aspects of animal nutrition, such as raw material evaluation, feed additives, nutritive value of novel ingredients and feed safety.