LncRNA TTC28-AS1 promotes the progression of cervical cancer by targeting miR-377-3p

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research Pub Date : 2025-06-10 DOI:10.1111/jog.16331

Nianhong Yang, Tingting Xia, Ying Huang

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引用次数: 0

Abstract

Aim

Cervical cancer (CC) is the fourth most common malignancy affecting women worldwide and has a poor prognosis. The aim of this study was to investigate the clinical significance and mechanism of TTC28-AS1 in CC.

Methods

A total of 120 individuals suffering from CC were included in this study. RT-qPCR was used to detect the level of TTC28-AS1, and the prognostic value of TTC28-AS1 was analyzed by Kaplan–Meier curve and Cox regression analyses. Dual-Luciferase reporter assay was used to verify the targeting relationship between TTC28-AS1 and miR-377-3p. The proliferative ability of CC cells was detected by CCK-8 kit. The migration and invasion of CC cells were detected by transwell system.

Results

The expression level of TTC28-AS1 was elevated in patients with CC, serving as an indicator of unfavorable clinical outcome. TTC28-AS1 may promote the proliferation, migration, and invasion of CC cells by targeting miR-377-3p.

Conclusions

TTC28-AS1 has the potential to promote the malignant progression of CC by targeting miR-377-3p, thus emerging as a promising therapeutic target for the treatment of CC.

查看原文本刊更多论文

LncRNA TTC28-AS1通过靶向miR-377-3p促进宫颈癌的进展

目的宫颈癌（CC）是影响妇女的第四大常见恶性肿瘤，预后较差。本研究旨在探讨TTC28-AS1在CC中的临床意义及作用机制。方法选取120例CC患者作为研究对象。采用RT-qPCR检测TTC28-AS1水平，采用Kaplan-Meier曲线和Cox回归分析TTC28-AS1的预后价值。采用双荧光素酶报告基因法验证TTC28-AS1与miR-377-3p之间的靶向关系。CCK-8试剂盒检测CC细胞的增殖能力。transwell系统检测CC细胞的迁移和侵袭。结果TTC28-AS1在CC患者中表达水平升高，可作为临床预后不良的指标。TTC28-AS1可能通过靶向miR-377-3p促进CC细胞的增殖、迁移和侵袭。结论TTC28-AS1有可能通过靶向miR-377-3p促进CC的恶性进展，从而成为治疗CC的一个有希望的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Obstetrics and Gynaecology Research 医学-妇产科学

CiteScore

3.10

自引率

0.00%

发文量

376

审稿时长

3-6 weeks

期刊介绍： The Journal of Obstetrics and Gynaecology Research is the official Journal of the Asia and Oceania Federation of Obstetrics and Gynecology and of the Japan Society of Obstetrics and Gynecology, and aims to provide a medium for the publication of articles in the fields of obstetrics and gynecology. The Journal publishes original research articles, case reports, review articles and letters to the editor. The Journal will give publication priority to original research articles over case reports. Accepted papers become the exclusive licence of the Journal. Manuscripts are peer reviewed by at least two referees and/or Associate Editors expert in the field of the submitted paper.